ArticleBMC biotechnology2007
Plastoglobules: a new address for targeting recombinant proteins in the chloroplast.
Article in BMC biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- A conserved sequence insert of fibrillins is involved in plastoglobule association and lipid binding.Plant & cell physiology · 2026Article
- Chemical modulation of chloroplast de- and redifferentiation reveals a role for the SAL1-PAP retrograde pathway in facilitating plastid transitions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A Chemical Probe for Increasing Leaf Tocopherol Levels by Coordinated Modulation of Biosynthesis, Competition and Storage.Plant biotechnology journal · 2026Article
- Chemical modulation of chloroplast de- and re-differentiation reveals a role for the SAL1-PAP retrograde pathway in facilitating plastid transitions.bioRxiv : the preprint server for biology · 2025Article
- Cyto-architecture of Byblis glands and leaf cells based on freeze-substitution and conventional TEM.Annals of botany · 2025Article
- GhCTSF1, a short PPR protein with a conserved role in chloroplast development and photosynthesis, participates in intron splicing of rpoC1 and ycf3-2 transcripts in cotton.Plant communications · 2024Article
- Identification of TaGL1-B1 gene controlling grain length through regulation of jasmonic acid in common wheat.Plant biotechnology journal · 2023Article
- Insights into topology and membrane interaction characteristics of plastoglobule-localized AtFBN1a and AtLOX2.Plant signaling & behavior · 2021Article
- Plastoglobular protein 18 is involved in chloroplast function and thylakoid formation.Journal of experimental botany · 2019Article
- Proteomic Analysis of Lipid Droplets from Arabidopsis Aging Leaves Brings New Insight into Their Biogenesis and Functions.Frontiers in plant science · 2017Article
- Plastoglobule-Targeting Competence of a Putative Transit Peptide Sequence from Rice Phytoene Synthase 2 in Plastids.International journal of molecular sciences · 2016Article
- Carotenoid Cleavage Oxygenases from Microbes and Photosynthetic Organisms: Features and Functions.International journal of molecular sciences · 2016Review
- The Role of the Atypical Kinases ABC1K7 and ABC1K8 in Abscisic Acid Responses.Frontiers in plant science · 2016Article
- Differential Subplastidial Localization and Turnover of Enzymes Involved in Isoprenoid Biosynthesis in Chloroplasts.PloS one · 2016Article
- Stress-induced chloroplast degradation in Arabidopsis is regulated via a process independent of autophagy and senescence-associated vacuoles.The Plant cell · 2014Article
- Mediated plastid RNA editing in plant immunity.PLoS pathogens · 2013Article
- When proteomics reveals unsuspected roles: the plastoglobule example.Frontiers in plant science · 2013Article
- Plastid localization of the key carotenoid enzyme phytoene synthase is altered by isozyme, allelic variation, and activity.The Plant cell · 2012Article
- Review
- The functional network of the Arabidopsis plastoglobule proteome based on quantitative proteomics and genome-wide coexpression analysis.Plant physiology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe potential of transgenic plants for cost-effective production of pharmaceutical molecules is now becoming apparent. Plants have the advantage over established fermentation systems (bacterial, yeast or animal cell cultures) to circumvent the risk of pathogen contamination, to be amenable to large scaling up and to necessitate only established farming procedures. Chloroplasts have proven a useful cellular compartment for protein accumulation owing to their large size and number, as well as the possibility for organellar transformation. They therefore represent the targeting destination of choice for recombinant proteins in leaf crops such as tobacco. Extraction and purification of recombinant proteins from leaf material contribute to a large extent to the production costs. Developing new strategies facilitating these processes is therefore necessary.
resultsHere, we evaluated plastoglobule lipoprotein particles as a new subchloroplastic destination for recombinant proteins. The yellow fluorescent protein as a trackable cargo was targeted to plastoglobules when fused to plastoglobulin 34 (PGL34) as the carrier. Similar to adipocyte differentiation related protein (ADRP) in animal cells, most of the protein sequence of PGL34 was necessary for targeting to lipid bodies. The recombinant protein was efficiently enriched in plastoglobules isolated by simple flotation centrifugation. The viability of plants overproducing the recombinant protein was not affected, indicating that plastoglobule targeting did not significantly impair photosynthesis or sugar metabolism.
conclusionOur data identify plastoglobules as a new targeting destination for recombinant protein in leaf crops. The wide-spread presence of plastoglobules and plastoglobulins in crop species promises applications comparable to those of transgenic oilbody-oleosin technology in molecular farming.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.