ArticleBMC genomics2007
Large-scale mapping of mutations affecting zebrafish development.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 67 citations in OpenAlex.
- Review
- De novo screening of disease-resistant genes from the chromosome-level genome of rare minnow using CRISPR-cas9 random mutation.GigaScience · 2021Article
- Zebrafish otolith biomineralization requires polyketide synthase.Mechanisms of development · 2019Article
- Endothelin receptor Aa regulates proliferation and differentiation of Erb-dependent pigment progenitors in zebrafish.PLoS genetics · 2019Article
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- Creation of miniature pig model of human Waardenburg syndrome type 2A by ENU mutagenesis.Human genetics · 2017Article
- Whole Genome Sequencing-Based Mapping and Candidate Identification of Mutations from Fixed Zebrafish Tissue.G3 (Bethesda, Md.) · 2017Article
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- Spermidine, but not spermine, is essential for pigment pattern formation in zebrafish.Biology open · 2016Article
- Otolith tethering in the zebrafish otic vesicle requires Otogelin and α-Tectorin.Development (Cambridge, England) · 2015Article
- Endothelin signalling in iridophore development and stripe pattern formation of zebrafish.Biology open · 2014Article
- Mirror movement-like defects in startle behavior of zebrafish dcc mutants are caused by aberrant midline guidance of identified descending hindbrain neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Article
- transparent, a gene affecting stripe formation in Zebrafish, encodes the mitochondrial protein Mpv17 that is required for iridophore survival.Biology open · 2013Article
- Physiology of SLC12 transporters: lessons from inherited human genetic mutations and genetically engineered mouse knockouts.American journal of physiology. Cell physiology · 2013Review
- RNA-seq-based mapping and candidate identification of mutations from forward genetic screens.Genome research · 2013Article
- The transcript catalogue of the short-lived fish Nothobranchius furzeri provides insights into age-dependent changes of mRNA levels.BMC genomics · 2013Article
- Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing.Development (Cambridge, England) · 2012Article
- Touch responsiveness in zebrafish requires voltage-gated calcium channel 2.1b.Journal of neurophysiology · 2012Article
- Efficient mapping and cloning of mutations in zebrafish by low-coverage whole-genome sequencing.Genetics · 2012Article
Corrections and comments
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Authors and funding
41 authors at 11 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLarge-scale mutagenesis screens in the zebrafish employing the mutagen ENU have isolated several hundred mutant loci that represent putative developmental control genes. In order to realize the potential of such screens, systematic genetic mapping of the mutations is necessary. Here we report on a large-scale effort to map the mutations generated in mutagenesis screening at the Max Planck Institute for Developmental Biology by genome scanning with microsatellite markers.
resultsWe have selected a set of microsatellite markers and developed methods and scoring criteria suitable for efficient, high-throughput genome scanning. We have used these methods to successfully obtain a rough map position for 319 mutant loci from the Tübingen I mutagenesis screen and subsequent screening of the mutant collection. For 277 of these the corresponding gene is not yet identified. Mapping was successful for 80 % of the tested loci. By comparing 21 mutation and gene positions of cloned mutations we have validated the correctness of our linkage group assignments and estimated the standard error of our map positions to be approximately 6 cM.
conclusionBy obtaining rough map positions for over 300 zebrafish loci with developmental phenotypes, we have generated a dataset that will be useful not only for cloning of the affected genes, but also to suggest allelism of mutations with similar phenotypes that will be identified in future screens. Furthermore this work validates the usefulness of our methodology for rapid, systematic and inexpensive microsatellite mapping of zebrafish mutations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.