ArticlePhotosynthesis research2006
Case study of combinatorial imaging: what protocol and what chlorophyll fluorescence image to use when visualizing infection of Arabidopsis thaliana by Pseudomonas syringae?
Article in Photosynthesis research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Leveraging chlorophyll fluorescence uncovers potato virus Y resistance in potato and its validation through viral quantification and yield loss studies.Plant cell reports · 2025Article
- Impact of Web Blight on Photosynthetic Performance of an Elite Common Bean Line in the Western Amazon Region of Colombia.Plants (Basel, Switzerland) · 2022Article
- Plant disease symptom segmentation in chlorophyll fluorescence imaging with a synthetic dataset.Frontiers in plant science · 2022Article
- Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry.Plants (Basel, Switzerland) · 2021Article
- Induction of a Compensatory Photosynthetic Response Mechanism in Tomato Leaves upon Short Time Feeding by the Chewing InsectInsects · 2021Article
- Image-Based Methods to Score Fungal Pathogen Symptom Progression and Severity in ExcisedPlants (Basel, Switzerland) · 2021Article
- Phenotyping Plant Responses to Biotic Stress by Chlorophyll Fluorescence Imaging.Frontiers in plant science · 2019Review
- Chlorophyll fluorescence imaging as a tool to monitor the progress of a root pathogen in a perennial plant.Planta · 2016Article
- ChlorophyllPlant methods · 2016Article
- Phenoplant: a web resource for the exploration of large chlorophyll fluorescence image datasets.Plant methods · 2015Article
- A Journey Through a Leaf: Phenomics Analysis of Leaf Growth in Arabidopsis thaliana.The arabidopsis book · 2015Article
- Image-based phenotyping of plant disease symptoms.Frontiers in plant science · 2014Article
- Chlorophyll fluorescence emission can screen cold tolerance of cold acclimated Arabidopsis thaliana accessions.Plant methods · 2014Article
- High throughput quantitative phenotyping of plant resistance using chlorophyll fluorescence image analysis.Plant methods · 2013Article
- Evidence for a role of raffinose in stabilizing photosystem II during freeze-thaw cycles.Planta · 2011Article
- Chlorophyll fluorescence emission as a reporter on cold tolerance in Arabidopsis thaliana accessions.Plant signaling & behavior · 2011Article
- Chlorophyll fluorescence imaging of plant-pathogen interactions.Protoplasma · 2010Review
- Towards discrimination of plant species by machine vision: advanced statistical analysis of chlorophyll fluorescence transients.Journal of fluorescence · 2009Article
- Early detection of bean infection by Pseudomonas syringae in asymptomatic leaf areas using chlorophyll fluorescence imaging.Photosynthesis research · 2008Article
- Constitutive and Regulatory Responses of Arabidopsis thaliana to Harmonically Oscillating Light.Physiologia plantarumArticle
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5 authors.
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Abstract
Localized infection of a plant can be mapped by a sequence of images capturing chlorophyll fluorescence transients in actinic light. Choice of the actinic light protocol co-determines fluorescence contrast between infected leaf segment and surrounding healthy tissue. Frequently, biology cannot predict with which irradiance protocol, in which fluorescence image of the sequence, and in which segment of the image there will be the highest contrast between the healthy and infected tissue. Here, we introduce a new technique that can be applied to identify the combination of chlorophyll fluorescence images yielding the highest contrast. The sets of the most contrasting images vary throughout the progress of the infection. Such specific image sets, stress-revealing fluorescence signatures, can be found for the initial and late phases of the infection. Using these signatures, images can be divided into segments that show tissue in different infection phases. We demonstrate the capacity of the algorithm in an investigation of infection of the model plant Arabidopsis thaliana by the bacterium Pseudomonas syringae. We show that the highest contrast is found with transients elicited by fluctuating, harmonically modulated irradiance with long periods.
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