ArticleThe Journal of cell biology2007
The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating.
Article in The Journal of cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- α/β Hydrolases: Toward Unraveling Entangled Classification.Proteins · 2025Article
- Deletion of theMicroorganisms · 2020Article
- Two novel effectors of trafficking and maturation of the yeast plasma membrane HTraffic (Copenhagen, Denmark) · 2017Article
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
- Identification and characterization of LFD-2, a predicted fringe protein required for membrane integrity during cell fusion in neurospora crassa.Eukaryotic cell · 2015Article
- Membrane organization and cell fusion during mating in fission yeast requires multipass membrane protein Prm1.Genetics · 2014Article
- Genetic basis of cell-cell fusion mechanisms.Trends in genetics : TIG · 2013Review
- Mate and fuse: how yeast cells do it.Open biology · 2013Review
- Serine-type carboxypeptidase KexA of Aspergillus oryzae has broader substrate specificity than Saccharomyces cerevisiae Kex1 and is required for normal hyphal growth and conidiation.Applied and environmental microbiology · 2012Article
- Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Prm1 functions as a disulfide-linked complex in yeast mating.The Journal of biological chemistry · 2010Article
- Article
- The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes.Genes & development · 2008Article
- Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast.The Journal of cell biology · 2008Article
- Function and regulation in MAPK signaling pathways: lessons learned from the yeast Saccharomyces cerevisiae.Biochimica et biophysica acta · 2007Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular machines that mediate cell fusion are unknown. Previously, we identified a multispanning transmembrane protein, Prm1 (pheromone-regulated membrane protein 1), that acts during yeast mating (Heiman, M.G., and P. Walter. 2000. J. Cell Biol. 151:719-730). Without Prm1, a substantial fraction of mating pairs arrest with their plasma membranes tightly apposed yet unfused. In this study, we show that lack of the Golgi-resident protease Kex2 strongly enhances the cell fusion defect of Prm1-deficient mating pairs and causes a mild fusion defect in otherwise wild-type mating pairs. Lack of the Kex1 protease but not the Ste13 protease results in similar defects. Deltakex2 and Deltakex1 fusion defects were suppressed by osmotic support, a trait shared with mutants defective in cell wall remodeling. In contrast, other cell wall mutants do not enhance the Deltaprm1 fusion defect. Electron microscopy of Deltakex2-derived mating pairs revealed novel extracellular blebs at presumptive sites of fusion. Kex2 and Kex1 may promote cell fusion by proteolytically processing substrates that act in parallel to Prm1 as an alternative fusion machine, as cell wall components, or both.
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