ArticleMolecular and cellular biology2007
Mechanism of histone H1-stimulated glucocorticoid receptor DNA binding in vivo.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Correlation among DNA Linker Length, Linker Histone Concentration, and Histone Tails in Chromatin.Biophysical journal · 2016Article
- Quantification of transcription factor-DNA binding affinity in a living cell.Nucleic acids research · 2016Article
- Germline-specific H1 variants: the "sexy" linker histones.Chromosoma · 2016Review
- The H1 linker histones: multifunctional proteins beyond the nucleosomal core particle.EMBO reports · 2015Review
- ICM Web: the interactive chromatin modeling web server.Nucleic acids research · 2010Article
- Histone H1 phosphorylation is associated with transcription by RNA polymerases I and II.The Journal of cell biology · 2010Article
- Characterization of somatic cell nuclear reprogramming by oocytes in which a linker histone is required for pluripotency gene reactivation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Androgen stimulates transcription and replication of xenotropic murine leukemia virus-related virus.Journal of virology · 2010Article
- FoxA1 binding directs chromatin structure and the functional response of a glucocorticoid receptor-regulated promoter.Molecular and cellular biology · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xenopus oocytes lack somatic linker histone H1 but contain an oocyte-specific variant, B4. The glucocorticoid receptor (GR) inducible mouse mammary tumor virus (MMTV) promoter was reconstituted in Xenopus oocytes to address the effects of histone H1. The expression of Xenopus H1o [corrected] (H1) via cytoplasmic mRNA injection resulted in H1 incorporation into in vivo assembled chromatin based on (i) the appearance of a chromatosome stop, (ii) the increased nucleosome repeat length (NRL), and (iii) H1-DNA binding assayed by chromatin immunoprecipitation (ChIP). The H1 effect on the NRL was saturable and hence represents H1-binding to a specific site. A subsaturating level of H1 enhanced the hormone-dependent binding of GR to the glucocorticoid response elements (GREs) and the hormone-dependent MMTV transcription while it reduced the access to DNA as revealed by micrococcal nuclease (MNase) analysis. These H1 effects were lost at higher levels of H1. ChIP and MNase analysis revealed a hormone-dependent dissociation of H1 from the activated chromatin domain. The proposed mechanism of H1-induced GR binding is based on two effects: (i) a GR-induced asymmetric distribution of H1 in favor of inactive chromatin and (ii) an H1-induced reduction in DNA access. These effects results in increased concentration of free GR and, hence, in increased GR-GRE binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.