ArticleFree radical biology & medicine2007
Selective protection of nuclear thioredoxin-1 and glutathione redox systems against oxidation during glucose and glutamine deficiency in human colonic epithelial cells.
Article in Free radical biology & medicine, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 72 citations in OpenAlex.
- Thioredoxin System Protein Expression in Carcinomas of the Pancreas, Distal Bile Duct, and Ampulla in the United Kingdom.Diseases (Basel, Switzerland) · 2024Article
- Low-dose vanadium pentoxide perturbed lung metabolism associated with inflammation and fibrosis signaling in male animal and in vitro models.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Firsthand and Secondhand Exposure Levels of Maltol-Flavored Electronic Nicotine Delivery System Vapors Disrupt Amino Acid Metabolism.Toxicological sciences : an official journal of the Society of Toxicology · 2021Article
- Extracellular vesicle-mediated macrophage activation: An insight into the mechanism of thioredoxin-mediated immune activation.Redox biology · 2019Article
- Redox Systems Biology of Nutrition and Oxidative Stress.The Journal of nutrition · 2019Article
- Glutathione depleting drugs, antioxidants and intestinal calcium absorption.World journal of gastroenterology · 2018Review
- Mitochondrial network responses in oxidative physiology and disease.Free radical biology & medicine · 2018Review
- Reactive Oxygen Species and NOX Enzymes Are Emerging as Key Players in Cutaneous Wound Repair.International journal of molecular sciences · 2017Review
- Redox theory of aging: implications for health and disease.Clinical science (London, England : 1979) · 2017Review
- The cysteine proteome.Free radical biology & medicine · 2015Review
- Integrated redox proteomics and metabolomics of mitochondria to identify mechanisms of cd toxicity.Toxicological sciences : an official journal of the Society of Toxicology · 2014Article
- Disturbed flow enhances inflammatory signaling and atherogenesis by increasing thioredoxin-1 level in endothelial cell nuclei.PloS one · 2014Article
- Reactive oxygen species and redox compartmentalization.Frontiers in physiology · 2014Review
- Nutritional countermeasures targeting reactive oxygen species in cancer: from mechanisms to biomarkers and clinical evidence.Antioxidants & redox signaling · 2013Review
- Selective targeting of the cysteine proteome by thioredoxin and glutathione redox systems.Molecular & cellular proteomics : MCP · 2013Article
- The interactions of oxidative stress and inflammation with vascular dysfunction in ageing: the vascular health triad.Age (Dordrecht, Netherlands) · 2013Review
- Annexin A2: the importance of being redox sensitive.International journal of molecular sciences · 2013Article
- Increased nuclear thioredoxin-1 potentiates cadmium-induced cytotoxicity.Toxicological sciences : an official journal of the Society of Toxicology · 2013Article
- Article
- In vitro susceptibility of thioredoxins and glutathione to redox modification and aging-related changes in skeletal muscle.Free radical biology & medicine · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
Little is known about the relative sensitivities of antioxidant systems in nuclei, mitochondria, and cytoplasm. The present study examined the oxidation of the thiol-dependent antioxidant systems in these subcellular compartments under conditions of limited energy supply of human colonic epithelial HT-29 cells induced by depletion of glucose (Glc) and glutamine (Gln) from the culture medium. Increased oxidation of dichlorofluoroscein (DCF) indicated an increased level of reactive oxygen species (ROS). Redox Western blot analysis showed oxidation of cytosolic thioredoxin-1 (Trx1) and mitochondrial thioredoxin-2 (Trx2) by 24 h, but little oxidation of nuclear Trx1. The Trx1 substrate, redox factor-1 (Ref-1), was also oxidized in cytosol but was reduced in nuclei. Protein S-glutathionylation (PrSSG), expressed as a ratio of protein thiol (PrSH), was also increased in the cytosol, while nuclear PrSSG/PrSH was not. Taken together, the data show that oxidative stress induced by depletion of Glc and Gln affects the redox states of proteins in the cytoplasm and mitochondria more than those in the nucleus. These results indicate that the nuclear compartment has better protection against oxidative stress than cytoplasm or mitochondria. These results further suggest that energy and/or substrate supply may contribute to sensitivity of mitochondrial and cytoplasmic systems to oxidative damage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.