ArticleJournal of periodontology2006
Characterization of minocycline transport by human neutrophils.
Article in Journal of periodontology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Pharmacokinetics of doxycycline in adults with cystic fibrosis.Antimicrobial agents and chemotherapy · 2012Trial
- In vitro antibiotic activity against intraosteoblastic Staphylococcus aureus: a narrative review of the literature.The Journal of antimicrobial chemotherapy · 2021Review
- Effects of 9-t-butyl doxycycline on the innate immune response to CNS ischemia-reperfusion injury.Experimental and molecular pathology · 2021Article
- Intracellular Penetration and Effects of Antibiotics onAntibiotics (Basel, Switzerland) · 2019Review
- Systemic antimicrobial therapy (minocycline) as an adjunct to non-surgical approach to recurrent chronic generalized gingival hyperplasia.Journal of Indian Society of Periodontology · 2014Article
- Use of minocycline as systemic antimicrobial therapy in refractory periodontitis with chronic gingival enlargement.Journal of advanced pharmaceutical technology & research · 2012Article
- Clarithromycin accumulation by phagocytes and its effect on killing of Aggregatibacter actinomycetemcomitans.Journal of periodontology · 2011Article
- Proteomic analysis of the anti-inflammatory action of minocycline.Proteomics · 2011Article
- Tetracyclines: a pleitropic family of compounds with promising therapeutic properties. Review of the literature.American journal of physiology. Cell physiology · 2010Review
- Cardiac uptake of minocycline and mechanisms for in vivo cardioprotection.Journal of the American College of Cardiology · 2008Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
backgroundTetracyclines are used in periodontal therapy as antimicrobial agents and as inhibitors of matrix metalloproteinases. Neutrophils appear to accumulate minocycline and other tetracyclines through a mechanism that has not been fully characterized.
methodsThe transport of minocycline and other tetracyclines by isolated human neutrophils was characterized by measuring the increase in cell-associated fluorescence.
resultsQuiescent neutrophils took up minocycline through a saturable, concentrative, sodium-dependent mechanism with a Michaelis constant (K(m)) of 153 micro g/ml (501 microM) and a maximal velocity of 240 ng/minute/10(6) cells. The efficiency of minocycline transport was not influenced significantly by a two-unit variation in extracellular pH and was not enhanced upon cell activation with phorbol myristate acetate. Neutrophil incubation in medium containing 10 micro g/ml minocycline, doxycycline, or tetracycline yielded steady-state intracellular/extracellular concentration ratios of approximately 64.0, 7.5, or 1.8, respectively. The dilution of extracellular minocycline or doxycycline triggered efflux from cells loaded with these antibiotics. Minocycline transport was competitively inhibited by the organic cations carnitine, diphenhydramine, and verapamil, but penicillin and other organic anions failed to produce inhibition.
conclusionTransport of tetracyclines by neutrophils could potentially enhance the effectiveness of these agents in periodontal therapy by enhancing or sustaining their therapeutic levels at inflammatory sites and by enhancing the killing of phagocytosed bacterial pathogens.
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Registered trials
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