ArticleThe Journal of biological chemistry2007
A conserved cysteine residue of Pichia pastoris Pex20p is essential for its recycling from the peroxisome to the cytosol.
Article in The Journal of biological chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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Who cites it
33 citing papers in PubMed, 87 citations in OpenAlex.
- Proteomic approaches to study ubiquitinomics.Biochimica et biophysica acta. Gene regulatory mechanisms · 2023Review
- An expanded lexicon for the ubiquitin code.Nature reviews. Molecular cell biology · 2023Review
- Deciphering non-canonical ubiquitin signaling: biology and methodology.Frontiers in molecular biosciences · 2023Review
- Article
- Insights into the Structure and Function of the Pex1/Pex6 AAA-ATPase in Peroxisome Homeostasis.Cells · 2022Review
- Non-lysine ubiquitylation: Doing things differently.Frontiers in molecular biosciences · 2022Review
- Post-translational modifications of proteins associated with yeast peroxisome membrane: An essential mode of regulatory mechanism.Genes to cells : devoted to molecular & cellular mechanisms · 2021Review
- The Targeting of Native Proteins to the Endoplasmic Reticulum-Associated Degradation (ERAD) Pathway: An Expanding Repertoire of Regulated Substrates.Biomolecules · 2021Review
- Comparative Genomics of Peroxisome Biogenesis Proteins: Making Sense of the PEX Proteins.Frontiers in cell and developmental biology · 2021Article
- The role of ubiquitination in tumorigenesis and targeted drug discovery.Signal transduction and targeted therapy · 2020Review
- The autophagic degradation of cytosolic pools of peroxisomal proteins by a new selective pathway.Autophagy · 2020Article
- Membrane Processing and Steady-State Regulation of the Alternative Peroxisomal Import Receptor Pex9p.Frontiers in cell and developmental biology · 2020Article
- Cellular functions and molecular mechanisms of non-lysine ubiquitination.Open biology · 2019Review
- Current Advances in Protein Import into Peroxisomes.The protein journal · 2019Review
- Receptor recognition by the peroxisomal AAA complex depends on the presence of the ubiquitin moiety and is mediated by Pex1p.The Journal of biological chemistry · 2018Article
- Translational and post-translational regulation of mouse cation transport regulator homolog 1.Scientific reports · 2016Article
- E2 enzymes: more than just middle men.Cell research · 2016Review
- Cysteine-specific ubiquitination protects the peroxisomal import receptor Pex5p against proteasomal degradation.Bioscience reports · 2015Article
- Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.Autophagy · 2015Article
- The unique degradation pathway of the PTS2 receptor, Pex7, is dependent on the PTS receptor/coreceptor, Pex5 and Pex20.Molecular biology of the cell · 2014Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
We identified a cysteine residue, conserved near the N terminus of Pex5p- and Pex20p-like proteins, that is essential for the cytosolic relocation of peroxisomal Pex20p. Surprisingly, this residue is not completely essential for the function of the protein; its point mutation into a serine in Pex20p(C8S) causes the accumulation of the protein at the peroxisome membrane, but this is quickly followed by its subsequent degradation by an ubiquitin-dependent quality control pathway called RADAR (receptor accumulation and degradation in the absence of recycling). This degradative pathway allows partial growth of the Pex20p(C8S) mutant on peroxisome-requiring medium. Mutation of cysteine 8 (C8S) and lysine 19 (K19R), the target residue of the RADAR pathway within Pex20p, leads to a stable but non-functional protein because it fails to recycle to the cytosol. This suggests a role for Cys-8 in Pex20p recycling and that constitutive degradation of peroxisomal receptors can be a partially functional alternative to receptor recycling. In addition, expression of this mutant protein in wild-type cells confers a dominant-negative, oleate-specific growth defect, which is a useful tool for a better understanding of peroxisomal receptor recycling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.