ArticleJournal of bacteriology2007
Amplification of lac cannot account for adaptive mutation to Lac+ in Escherichia coli.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Redox stress agents strongly enhance mutagenesis during horizontal gene transfer in bacteria and leave distinct mutational and metabolic footprints.bioRxiv : the preprint server for biology · 2026Article
- Efficient mathematical methodology to determine multistep mutant burden in spatially growing cell populations.PNAS nexus · 2025Article
- Quantum aspects of evolution: a contribution towards evolutionary explorations of genotype networks via quantum walks.Journal of the Royal Society, Interface · 2020Article
- Selection and Plasmid Transfer Underlie Adaptive Mutation inGenetics · 2018Article
- Interactions and Localization of Escherichia coli Error-Prone DNA Polymerase IV after DNA Damage.Journal of bacteriology · 2015Article
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- Effect of growth under selection on appearance of chromosomal mutations in Salmonella enterica.Genetics · 2011Article
- Impact of a stress-inducible switch to mutagenic repair of DNA breaks on mutation in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The SMC-like protein complex SbcCD enhances DNA polymerase IV-dependent spontaneous mutation in Escherichia coli.Journal of bacteriology · 2011Article
- The sigma(E) stress response is required for stress-induced mutation and amplification in Escherichia coli.Molecular microbiology · 2010Article
- Expansion of a chromosomal repeat in Escherichia coli: roles of replication, repair, and recombination functions.BMC molecular biology · 2009Article
- Role of polyphosphates in microbial adaptation to extreme environments.Applied and environmental microbiology · 2008Review
- The Escherichia coli histone-like protein HU has a role in stationary phase adaptive mutation.Genetics · 2007Article
- Article
- Mutation as a stress response and the regulation of evolvability.Critical reviews in biochemistry and molecular biologyReview
- Stress-induced mutagenesis in bacteria.Critical reviews in biochemistry and molecular biologyReview
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
When the Lac- strain of Escherichia coli, FC40, is incubated with lactose as its sole carbon and energy source, Lac+ revertants arise at a constant rate, a phenomenon known as adaptive mutation. Two alternative models for adaptive mutation have been proposed: (i) recombination-dependent mutation, which specifies that recombination occurring in nongrowing cells stimulates error-prone DNA synthesis, and (ii) amplification-dependent mutation, which specifies that amplification of the lac region and growth of the amplifying cells creates enough DNA replication to produce mutations at the normal rate. Here, we examined several of the predictions of the amplification-dependent mutation model and found that they are not fulfilled. First, inhibition of adaptive mutation by a gene that is toxic when overexpressed does not depend on the proximity of the gene to lac. Second, mutation at a second locus during selection for Lac+ revertants is also independent of the proximity of the locus to lac. Third, mutation at a second locus on the episome occurs even when the lac allele under selection is on the chromosome. Our results support the hypothesis that most Lac+ mutants that appear during lactose selection are true revertants that arise in a single step from Lac- cells, not from a population of growing or amplifying precursor cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.