ArticleJournal of bacteriology2007
S-layer anchoring and localization of an S-layer-associated protease in Caulobacter crescentus.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Prolific S-layer shedding and associated proteins from the methanotrophApplied and environmental microbiology · 2026Article
- Assembly, architecture and functional roles of microbial surface layers.Nature reviews. Microbiology · 2026Review
- Complete genome sequence ofMicrobiology resource announcements · 2026Article
- Cohesive Living Bacterial Films with Tunable Mechanical Properties from Cell Surface Protein Display.ACS synthetic biology · 2024Article
- Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.Nature communications · 2024Article
- Harnessing Innate Immunity to TreatCells · 2023Article
- What Glues the Glue to the Cell Surface?Journal of bacteriology · 2022Article
- A de novo matrix for macroscopic living materials from bacteria.Nature communications · 2022Article
- The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-OxidizingFrontiers in microbiology · 2021Article
- Article
- A bacterial surface layer protein exploits multistep crystallization for rapid self-assembly.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Topologically-guided continuous protein crystallization controls bacterial surface layer self-assembly.Nature communications · 2019Article
- Genomic Characterization of Candidate Division LCP-89 Reveals an Atypical Cell Wall Structure, Microcompartment Production, and Dual Respiratory and Fermentative Capacities.Applied and environmental microbiology · 2019Article
- Mechanisms of Resistance to the Contact-Dependent Bacteriocin CdzC/D inJournal of bacteriology · 2019Article
- An N-Terminal Retention Module Anchors the Giant Adhesin LapA of Pseudomonas fluorescens at the Cell Surface: a Novel Subfamily of Type I Secretion Systems.Journal of bacteriology · 2018Article
- Environmental Calcium Controls Alternate Physical States of the Caulobacter Surface Layer.Biophysical journal · 2017Article
- Structure of the hexagonal surface layer on Caulobacter crescentus cells.Nature microbiology · 2017Article
- Contact-dependent killing byeLife · 2017Article
- Surface-layer protein from Caulobacter crescentus: expression, purification and X-ray crystallographic analysis.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Bioadsorption of Rare Earth Elements through Cell Surface Display of Lanthanide Binding Tags.Environmental science & technology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The S-layer of the gram-negative bacterium Caulobacter crescentus is composed of a single protein, RsaA, that is secreted and assembled into a hexagonal crystalline array that covers the organism. Despite the widespread occurrence of comparable bacterial S-layers, little is known about S-layer attachment to cell surfaces, especially for gram-negative organisms. Having preliminary indications that the N terminus of RsaA anchors the monomer to the cell surface, we developed an assay to distinguish direct surface attachment from subunit-subunit interactions where small RsaA fragments are incubated with S-layer-negative cells to assess the ability of the fragments to reattach. In doing so, we found that the RsaA anchoring region lies in the first approximately 225 amino acids and that this RsaA anchoring region requires a smooth lipopolysaccharide species found in the outer membrane. By making mutations at six semirandom sites, we learned that relatively minor perturbations within the first approximately 225 amino acids of RsaA caused loss of anchoring. In other studies, we confirmed that only this N-terminal region has a direct role in S-layer anchoring. As a by-product of the anchoring studies, we discovered that Sap, the C. crescentus S-layer-associated protease, recognized a cleavage site in the truncated RsaA fragments that is not detected by Sap in full-length RsaA. This, in turn, led to the discovery that Sap was an extracellular membrane-bound protease, rather than intracellular, as previously proposed. Moreover, Sap was secreted to the cell surface primarily by the S-layer type I secretion apparatus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.