ArticleJournal of bacteriology2007
The cymA gene, encoding a tetraheme c-type cytochrome, is required for arsenate respiration in Shewanella species.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 85 citations in OpenAlex.
- Metabolic diversity of Ferrovaceae and potential contributions to iron oxidation.Applied and environmental microbiology · 2026Article
- From genes to function: regulation, maturation, and evolution of cytochromeApplied and environmental microbiology · 2025Review
- Novel Insights on Extracellular Electron Transfer Networks in theMicroorganisms · 2024Article
- Review
- Characterization of the inner membrane cytochrome ImcH from Geobacter reveals its importance for extracellular electron transfer and energy conservation.Protein science : a publication of the Protein Society · 2023Article
- Looking for the mechanism of arsenate respiration ofFrontiers in microbiology · 2022Article
- Improving Arsenic Tolerance of Pyrococcus furiosus by Heterologous Expression of a Respiratory Arsenate Reductase.Applied and environmental microbiology · 2020Article
- Genetic mechanisms of arsenic detoxification and metabolism in bacteria.Current genetics · 2019Review
- Structural and mechanistic analysis of the arsenate respiratory reductase provides insight into environmental arsenic transformations.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Genomic Analyses of the Quinol Oxidases and/or Quinone Reductases Involved in Bacterial Extracellular Electron Transfer.Frontiers in microbiology · 2018Article
- Extracellular electron transport-mediated Fe(III) reduction by a community of alkaliphilic bacteria that use flavins as electron shuttles.Applied and environmental microbiology · 2014Article
- Evaluative profiling of arsenic sensing and regulatory systems in the human microbiome project genomes.Microbiology insights · 2014Article
- Combined genomics and experimental analyses of respiratory characteristics of Shewanella putrefaciens W3-18-1.Applied and environmental microbiology · 2013Article
- The regulatory role of ferric uptake regulator (Fur) during anaerobic respiration of Shewanella piezotolerans WP3.PloS one · 2013Article
- Menaquinone-7 is specific cofactor in tetraheme quinol dehydrogenase CymA.The Journal of biological chemistry · 2012Article
- Dissimilatory reduction of extracellular electron acceptors in anaerobic respiration.Applied and environmental microbiology · 2012Review
- Molecular Underpinnings of Fe(III) Oxide Reduction by Shewanella Oneidensis MR-1.Frontiers in microbiology · 2012Article
- Article
- Physiological and evolutionary studies of NAP systems in Shewanella piezotolerans WP3.The ISME journal · 2011Article
- Recent advances in bacterial heme protein biochemistry.Current opinion in chemical biology · 2011Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Shewanella sp. strain ANA-3, utilization of arsenate as a terminal electron acceptor is conferred by a two-gene operon, arrAB, which lacks a gene encoding a membrane-anchoring subunit for the soluble ArrAB protein complex. Analysis of the genome sequence of Shewanella putrefaciens strain CN-32 showed that it also contained the same arrAB operon with 100% nucleotide identity. Here, we report that CN-32 respires arsenate and that this metabolism is dependent on arrA and an additional gene encoding a membrane-associated tetraheme c-type cytochrome, cymA. Deletion of cymA in ANA-3 also eliminated growth on and reduction of arsenate. The DeltacymA strains of CN-32 and ANA-3 negatively affected the reduction of Fe(III) and Mn(IV) but not growth on nitrate. Unlike the CN-32 DeltacymA strain, growth on fumarate was absent in the DeltacymA strain of ANA-3. Both homologous and heterologous complementation of cymA in trans restored growth on arsenate in DeltacymA strains of both CN-32 and ANA-3. Transcription patterns of cymA showed that it was induced under anaerobic conditions in the presence of fumarate and arsenate. Nitrate-grown cells exhibited the greatest level of cymA expression in both wild-type strains. Lastly, site-directed mutagenesis of the first Cys to Ser in each of the four CXXCH c-heme binding motifs of the CN-32 CymA nearly eliminated growth on and reduction of arsenate. Together, these results indicate that the biochemical mechanism of arsenate respiration and reduction requires the interactions of ArrAB with a membrane-associated tetraheme cytochrome, which in the non-arsenate-respiring Shewanella species Shewanella oneidensis strain MR-1, has pleiotropic effects on Fe(III), Mn(IV), dimethyl sulfoxide, nitrate, nitrite, and fumarate respiration.
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