ArticleJournal of bacteriology2007
Role of an FtsK-like protein in genetic stability in Streptomyces coelicolor A3(2).
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes.World journal of microbiology & biotechnology · 2022Article
- Influence of core divisome proteins on cell division inMicrobiology (Reading, England) · 2021Article
- Compaction and control-the role of chromosome-organizing proteins in Streptomyces.FEMS microbiology reviews · 2020Review
- Subtelomeres are fast-evolving regions of theMicrobial genomics · 2019Article
- A Waking Review: Old and Novel Insights into the Spore Germination inFrontiers in microbiology · 2017Review
- Developmental transcriptome of resting cell formation in Mycobacterium smegmatis.BMC genomics · 2016Article
- Article
- Developmental biology of Streptomyces from the perspective of 100 actinobacterial genome sequences.FEMS microbiology reviews · 2014Review
- Article
- Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation.Nucleic acids research · 2013Article
- Genome plasticity and systems evolution in Streptomyces.BMC bioinformatics · 2012Article
- Signals and regulators that govern Streptomyces development.FEMS microbiology reviews · 2012Review
- New insights on the development ofCurrent trends in microbiology · 2012Article
- A septal chromosome segregator protein evolved into a conjugative DNA-translocator protein.Mobile genetic elements · 2011Article
- Membrane-associated DNA transport machines.Cold Spring Harbor perspectives in biology · 2010Review
- One of the two genes encoding nucleoid-associated HU proteins in Streptomyces coelicolor is developmentally regulated and specifically involved in spore maturation.Journal of bacteriology · 2009Article
- cmdABCDEF, a cluster of genes encoding membrane proteins for differentiation and antibiotic production in Streptomyces coelicolor A3(2).BMC microbiology · 2009Article
- Genetic interactions of smc, ftsK, and parB genes in Streptomyces coelicolor and their developmental genome segregation phenotypes.Journal of bacteriology · 2009Article
- Streptomyces morphogenetics: dissecting differentiation in a filamentous bacterium.Nature reviews. Microbiology · 2009Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptomyces coelicolor A3(2) does not have a canonical cell division cycle during most of its complex life cycle, yet it contains a gene (ftsK(SC)) encoding a protein similar to FtsK, which couples the completion of cell division and chromosome segregation in unicellular bacteria such as Escherichia coli. Here, we show that various constructed ftsK(SC) mutants all grew apparently normally and sporulated but upon restreaking gave rise to many aberrant colonies and to high frequencies of chloramphenicol-sensitive mutants, a phenotype previously associated with large terminal deletions from the linear chromosome. Indeed, most of the aberrant colonies had lost large fragments near one or both chromosomal termini, as if chromosome ends had failed to reach their prespore destination before the closure of sporulation septa. A constructed FtsK(SC)-enhanced green fluorescent protein fusion protein was particularly abundant in aerial hyphae, forming distinctive complexes before localizing to each sporulation septum, suggesting a role for FtsK(SC) in chromosome segregation during sporulation. Use of a fluorescent reporter showed that when ftsK(SC) was deleted, several spore compartments in most spore chains failed to express the late-sporulation-specific sigma factor gene sigF, even though they contained chromosomal DNA. This suggested that sigF expression is autonomously activated in each spore compartment in response to completion of chromosome transfer, which would be a previously unknown checkpoint for late-sporulation-specific gene expression. These results provide new insight into the genetic instability prevalent among streptomycetes, including those used in the industrial production of antibiotics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.