ArticleBiophysical journal2007
Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl- pump activity.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 53 citations in OpenAlex.
- Molecular Biology of Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Unique ClBiophysics and physicobiology · 2021Article
- Functional Mechanism of ClAdvances in experimental medicine and biology · 2021Review
- Light-Driven Chloride Transport Kinetics of Halorhodopsin.Biophysical journal · 2018Article
- Electrical properties, substrate specificity and optogenetic potential of the engineered light-driven sodium pump eKR2.Scientific reports · 2018Article
- Conversion of a light-driven proton pump into a light-gated ion channel.Scientific reports · 2015Article
- Crystal structures of the L1, L2, N, and O states of pharaonis halorhodopsin.Biophysical journal · 2015Article
- Functional characterization of 5-oxoproline transport via SLC16A1/MCT1.The Journal of biological chemistry · 2015Article
- Structure-guided transformation of channelrhodopsin into a light-activated chloride channel.Science (New York, N.Y.) · 2014Article
- Biodesalination: a case study for applications of photosynthetic bacteria in water treatment.Plant physiology · 2014Review
- Ser(262) determines the chloride-dependent colour tuning of a new halorhodopsin from Haloquadratum walsbyi.Bioscience reports · 2012Article
- Homotrimer formation and dissociation of pharaonis halorhodopsin in detergent system.Biophysical journal · 2012Article
- Genetically encoded molecular tools for light-driven silencing of targeted neurons.Progress in brain research · 2012Review
- A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane proteins.Nature methods · 2011Article
- Ectopic expression of multiple microbial rhodopsins restores ON and OFF light responses in retinas with photoreceptor degeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- The mechanism of photo-energy storage in the Halorhodopsin chloride pump.The Journal of biological chemistry · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natronomonas pharaonis halorhodopsin (pHR) is an archaeal rhodopsin functioning as an inward-directed, light-driven Cl- pump. To characterize the electrophysiological features of the Cl- pump activity of pHR, we expressed pHR in Xenopus laevis oocytes and analyzed its photoinduced Cl- pump activity using the two-electrode voltage-clamp technique. Photoinduced outward currents were observed only in the presence of Cl-, Br-, I-, NO3-, and SCN-, but not in control oocytes, indicating that photoinduced anion currents were mediated by pHR. The relationship between photoinduced Cl- current via pHR and the light intensity was linear, demonstrating that transport of Cl- is driven by a single-photon reaction and that the steady-state current is proportional to the excited pHR molecule. The current-voltage relationship for pHR-mediated photoinduced currents was also linear between -150 mV and +50 mV. The slope of the line describing the current-voltage relationship increased as the number of the excited pHR molecules was increased by the light intensity. The reversal potential (VR) for Cl- as the substrate for the anion pump activity of pHR was about -400 mV. The value for VR was independent of light intensity, meaning that the VR reflects the intrinsic value of the excited pHR molecule. The value of VR changed significantly for the R123K mutant of pHR. We also show that the Cl- pump activity of pHR can generate a substantial negative membrane potential, indicating that pHR is a very potent Cl- pump. We have also analyzed the kinetics of voltage-dependent Cl- pump activity as well as that of the photocycle. Based on these data, a kinetic model for voltage-dependent Cl- transport via pHR is presented.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.