ArticlePlant physiology2007
Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 91 citations in OpenAlex.
- Ancient duplication and functional differentiation of phytochelatin synthases is conserved in plant genomes.Horticulture research · 2025Article
- Phytochelatin Synthase: An In Silico Comparative Analysis in Cyanobacteria and Eukaryotic Microalgae.Plants (Basel, Switzerland) · 2024Article
- Population Structure, Genetic Diversity and Candidate Genes for the Adaptation to Environmental Stress inPlants (Basel, Switzerland) · 2023Article
- Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants.International journal of molecular sciences · 2023Review
- Detoxifying the heavy metals: a multipronged study of tolerance strategies against heavy metals toxicity in plants.Frontiers in plant science · 2023Review
- Crosstalk and gene expression in microorganisms under metals stress.Archives of microbiology · 2022Review
- Stress-regulated elements inPeerJ · 2021Article
- Overexpression ofFrontiers in plant science · 2021Article
- Glutathione Restores Hg-Induced Morpho-Physiological Retardations by Inducing Phytochelatin and Oxidative Defense in Alfalfa.Biology · 2020Article
- Evolution and functional differentiation of recently diverged phytochelatin synthase genes from Arundo donax L.Journal of experimental botany · 2019Article
- Oxidative and genotoxic damages in plants in response to heavy metal stress and maintenance of genome stability.Plant signaling & behavior · 2018Article
- Traversing the Links between Heavy Metal Stress and Plant Signaling.Frontiers in plant science · 2018Review
- Overexpression of a FunctionalFrontiers in plant science · 2018Article
- Identification of alternatively spliced transcripts of rice phytochelatin synthase 2 gene OsPCS2 involved in mitigation of cadmium and arsenic stresses.Plant molecular biology · 2017Article
- Horizontal Gene Transfer of Phytochelatin Synthases from Bacteria to Extremophilic Green Algae.Microbial ecology · 2017Article
- Transcriptome Profiling of Louisiana iris Root and Identification of Genes Involved in Lead-Stress Response.International journal of molecular sciences · 2015Article
- Heavy Metal Tolerance in Plants: Role of Transcriptomics, Proteomics, Metabolomics, and Ionomics.Frontiers in plant science · 2015Review
- Transcriptional and physiological changes in relation to Fe uptake under conditions of Fe-deficiency and Cd-toxicity in roots of Vigna radiata L.Journal of plant research · 2014Article
- Arabidopsis thaliana phytochelatin synthase 2 is constitutively active in vivo and can rescue the growth defect of the PCS1-deficient cad1-3 mutant on Cd-contaminated soil.Journal of experimental botany · 2014Article
- Thiol-based redox signaling in the nitrogen-fixing symbiosis.Frontiers in plant science · 2013Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biosynthesis of phytochelatins and homophytochelatins has been studied in nodulated plants of the model legume Lotus (Lotus japonicus). In the first 6 to 24 h of treatment with cadmium (Cd), roots started to synthesize elevated amounts of both polypeptides, with a concomitant increase of glutathione and a decrease of homoglutathione, indicating the presence of active phytochelatin synthase (PCS) genes. Screening of transformation-competent artificial chromosome libraries allowed identification of a cluster of three genes, LjPCS1, LjPCS2, and LjPCS3, which were mapped at 69.0 cM on chromosome 1. The genes differ in exon-intron composition and responsiveness to Cd. Gene structures and phylogenetic analysis of the three protein products, LjPCS1-8R, LjPCS2-7N, and LjPCS3-7N, are consistent with two sequential gene duplication events during evolution of vascular plants. Two sites for alternative splicing in the primary transcripts were identified. One of them, involving intron 2 of the LjPCS2 gene, was confirmed by the finding of the two predicted mRNAs, encoding LjPCS2-7R in roots and LjPCS2-7N in nodules. The amino acid sequences of LjPCS2-7R (or LjPCS2-7N) and LjPCS3-7N share 90% identity, but have only 43% to 59% identity with respect to the typical PCS1 enzymes of Lotus and other plants. The unusual LjPCS2-7N and LjPCS3-7N proteins conferred Cd tolerance when expressed in yeast (Saccharomyces cerevisiae) cells, whereas the alternatively spliced form, LjPCS2-7R, differing only in a five-amino acid motif (GRKWK) did not. These results unveil complex regulatory mechanisms of PCS expression in legume tissues in response to heavy metals and probably to other developmental and environmental factors.
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