ArticleMolecular and cellular biochemistry2007
Osteogenic differentiation of adipose derived stem cells promoted by overexpression of osterix.
Article in Molecular and cellular biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- circRNA422 enhanced osteogenic differentiation of bone marrow mesenchymal stem cells during early osseointegration through the SP7/LRP5 axis.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Hsa_circ_0099630 knockdown induces the proliferation and osteogenic differentiation and attenuates the apoptosis of porphyromonas gingivalis lipopolysaccharide-induced human periodontal ligament fibroblasts.Annals of translational medicine · 2022Article
- Preclinical Evaluation of BMP-9-Treated Human Bone-like Substitutes for Alveolar Ridge Preservation following Tooth Extraction.International journal of molecular sciences · 2022Article
- Recent Advances of Osterix Transcription Factor in Osteoblast Differentiation and Bone Formation.Frontiers in cell and developmental biology · 2020Review
- Extracellular Matrices to Modulate the Innate Immune Response and Enhance Bone Healing.Frontiers in immunology · 2019Review
- Targeting sphingosine-1-phosphate lyase as an anabolic therapy for bone loss.Nature medicine · 2018Article
- Restoration of osteogenic differentiation by overexpression of cannabinoid receptor 2 in bone marrow mesenchymal stem cells isolated from osteoporotic patients.Experimental and therapeutic medicine · 2018Article
- Effects of Sr-HT-Gahnite on osteogenesis and angiogenesis by adipose derived stem cells for critical-sized calvarial defect repair.Scientific reports · 2017Article
- Dioscin stimulates differentiation of mesenchymal stem cells towards hypertrophic chondrocytesAmerican journal of translational research · 2016Article
- Activation of cannabinoid receptor 2 enhances osteogenic differentiation of bone marrow derived mesenchymal stem cells.BioMed research international · 2015Article
- Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis.Journal of Zhejiang University. Science. B · 2014Article
- The roles of bone morphogenetic proteins and their signaling in the osteogenesis of adipose-derived stem cells.Tissue engineering. Part B, Reviews · 2014Review
- Modulation of osteoblastic/odontoblastic differentiation of adult mesenchymal stem cells through gene introduction: a brief review.Journal of the Korean Association of Oral and Maxillofacial Surgeons · 2013Review
- Comparing the gene expression profile of stromal cells from human cord blood and bone marrow: lack of the typical "bone" signature in cord blood cells.Stem cells international · 2013Article
- Secreted factors from adipose tissue increase adipogenic differentiation of mesenchymal stem cells.Cell proliferation · 2012Article
- The Utility of Allograft Mesenchymal Stem Cells for Spine Fusion: A Literature Review.Global spine journal · 2012Review
- Article
- Adipose-derived stem cells in functional bone tissue engineering: lessons from bone mechanobiology.Tissue engineering. Part B, Reviews · 2011Review
- Dynamic expression of Runx2, Osterix and AJ18 in the femoral head of steroid-induced osteonecrosis in rats.Orthopaedic surgery · 2010Article
- Isolation and in vitro characterisation of dental pulp stem cells from natal teeth.Histochemistry and cell biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adipose-derived stem cells (ASCs) are considered to be multipotent mesenchymal stem cells that are easily induced to differentiate into functional osteoblasts both in vitro and in vivo. Osterix (Osx) is a zinc finger-containing transcription factor of Sp gene family, which plays important roles in bone development and mineralization. In this study, we hypothesized that overexpression of Osx in murine ASCs would promote their osteogenic differentiation in vitro. A plasmid expressing Osx (pcDNA3.1-Osx) was constructed and applied to transfect monolayers of murine ASCs. Then expression of bone-related genes, nodule formation, proliferation rate, and alkaline phosphatase activity were examined to evaluate the osteogenic potential of ASCs with pcDNA3.1-Osx transfection. Results of RT-PCR and immunohistochemistry showed that pcDNA3.1-Osx transfection enhanced the expression of bone matrix proteins, such as bone sialoprotein, osteocalcin, osteopontin, and Collagen type I in ASCs. At the same time, overexpression of Osx in ASCs enhanced alkaline phosphatase activity and capability to form mineralized nodules, while not inhibited their proliferation rate. These results indicated that pcDNA3.1-Osx transfection promoted the osteogenic differentiation of ASCs, while not affecting their proliferative ability. Since they can be easily isolated and genetically modified, ASCs are hopeful cell sources in the further application of hard tissue engineering.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.