ArticleProteomics2007
Proteomic profiling identifies breast tumor metastasis-associated factors in an isogenic model.
Article in Proteomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer.Cell death & disease · 2018Article
- Nuclear repartitioning of galectin-1 by an extracellular glycan switch regulates mammary morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer.Molecular & cellular proteomics : MCP · 2015Article
- Article
- An optimized isolation of biotinylated cell surface proteins reveals novel players in cancer metastasis.Journal of proteomics · 2012Article
- Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis.Oncogene · 2011Article
- Differential protein expression analysis using stable isotope labeling and PQD linear ion trap MS technology.Journal of the American Society for Mass Spectrometry · 2009Article
- Do serum biomarkers really measure breast cancer?BMC cancer · 2009Article
- Breast tumor metastasis: analysis via proteomic profiling.Expert review of proteomics · 2008Review
- Bladder cancer associated glycoprotein signatures revealed by urinary proteomic profiling.Journal of proteome research · 2007Article
- Differential expression of ribosomal proteins in a human metastasis model identified by coupling 2-D liquid chromatography and mass spectrometry.Cancer genomics & proteomicsArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
A combination of LC and MS was applied to an isogenic breast tumor metastasis model to identify proteins associated with a cellular phenotype. Chromatofocusing followed by nonporous-RP-HPLC/ESI-TOF MS was applied to cell lysates of a pair of monoclonal cell lines from the human breast carcinoma cell line MDA-MB-435 that have different metastatic phenotypes in immune-compromised mice. This method was developed to separate proteins based on pI and hydrophobicity. The high resolution and mass accuracy of ESI-TOF measurements provided a good correlation of theoretical MW and experimental Mr values of intact proteins measured in mass maps obtained in the pH range 3.8-6.4. The isolated proteins were digested by trypsin and analyzed by MALDI-TOF MS, MALDI-QIT-TOF MS, and monolith-based HPLC/MS/MS. The unique combination of the techniques provided valuable information including quantitation and modification of proteins. We identified 89 selected proteins, of which 43 were confirmed as differentially expressed. Metastasis-associated proteins included galectin-1, whereas annexin I and annexin II were associated with the nonmetastatic phenotype. In this study, we demonstrate that combining a variety of MS tools with a multidimensional liquid-phase separation provides the ability to map cellular protein content, to search for modified proteins, and to correlate protein expression with cellular phenotype.
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Registered trials
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