ArticleProceedings of the National Academy of Sciences of the United States of America1991
Proteolysis patterns of epitopically labeled yeast DNA topoisomerase II suggest an allosteric transition in the enzyme induced by ATP binding.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- MeCP2 represses the activity of topoisomerase IIβ in long neuronal genes.Cell reports · 2023Article
- Negative supercoils regulate meiotic crossover patterns in budding yeast.Nucleic acids research · 2022Article
- Direct monitoring of the strand passage reaction of DNA topoisomerase II triggers checkpoint activation.PLoS genetics · 2013Article
- Review
- Analysis of the eukaryotic topoisomerase II DNA gate: a single-molecule FRET and structural perspective.Nucleic acids research · 2009Review
- Topoisomerase II: a fitted mechanism for the chromatin landscape.Nucleic acids research · 2009Review
- Article
- DNA topoisomerase II, genotoxicity, and cancer.Mutation research · 2007Review
- C-terminal regions of topoisomerase IIalpha and IIbeta determine isoform-specific functioning of the enzymes in vivo.Nucleic acids research · 2007Article
- mAMSA resistant human topoisomerase IIbeta mutation G465D has reduced ATP hydrolysis activity.Nucleic acids research · 2006Article
- Functional dissection of the C-terminal domain of type II DNA topoisomerase from the kinetoplastid hemoflagellate Leishmania donovani.Nucleic acids research · 2003Article
- Importance of the fourth alpha-helix within the CAP homology domain of type II topoisomerase for DNA cleavage site recognition and quinolone action.Antimicrobial agents and chemotherapy · 2002Article
- Protein footprinting at cysteines: probing ATP-modulated contacts in cysteine-substitution mutants of yeast DNA topoisomerase II.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Structural similarities between topoisomerases that cleave one or both DNA strands.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Modulation of gyrase-mediated DNA cleavage and cell killing by ATP.Antimicrobial agents and chemotherapy · 1998Article
- Proteolytic mapping of heat shock transcription factor domains.Protein science : a publication of the Protein Society · 1996Article
- Pat1: a topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiae.Nucleic acids research · 1996Article
- I(kappa)B(gamma) inhibits DNA binding of NF-kappaB p50 homodimers by interacting with residues that contact DNA.Molecular and cellular biology · 1996Article
- Analysis of functional domain organization in DNA topoisomerase II from humans and Saccharomyces cerevisiae.Molecular and cellular biology · 1996Article
- Structure and conformational changes of DNA topoisomerase II visualized by electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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2 authors.
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Abstract
A cloned yeast TOP2 gene was modified to produce yeast DNA topoisomerase II (EC 5.99.1.3) epitopically labeled at its amino or carboxyl terminus. Limited digestion with SV8 endoprotease shows three distinct protease-sensitive sites in each polypeptide of the dimeric enzyme. These sites were mapped by immunostaining of the end-labeled proteolytic fragments resolved by SDS/polyacrylamide gel electrophoresis; two of the mapped locations were confirmed by sequencing the amino ends of two unlabeled peptic fragments. Proteolytic cleavage by SV8 endoprotease at a pair of sites corresponding to the carboxyl sides of Glu-411 and Glu-680 is modulated by the binding of the nonhydrolyzable ATP analogs adenosine 5'-[beta, gamma-imido]triphosphate (5'-adenylyl imidodiphosphate) and adenosine 5'-[gamma-thio]triphosphate: in their absence cleavage occurs predominantly at Glu-411; in the presence of either analog, cleavage occurs predominantly at Glu-680. These results are interpreted in terms of allosteric interdomainal movements in the type II DNA topoisomerase following the binding of ATP.
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