ArticlePlant cell reports2007
Agrobacterium-mediated transformation of protocorm-like bodies in Cymbidium.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Article
- Breeding of ornamental orchids with focus on Phalaenopsis: current approaches, tools, and challenges for this century.Heredity · 2024Review
- Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update.Plants (Basel, Switzerland) · 2023Review
- Sex-Related Gene Network Revealed by Transcriptome Differentiation of Bisexual and Unisexual Flowers of OrchidInternational journal of molecular sciences · 2023Article
- Creating novel ornamentalsFrontiers in plant science · 2023Review
- Low Concentration of Anti-Auxin and Anti-Fungal Agent Accelerates the PLB Regeneration ofPlants (Basel, Switzerland) · 2022Article
- Ovule Development andInternational journal of molecular sciences · 2020Article
- Frequency, Spectrum, and Stability of Leaf Mutants Induced by Diverse γ-Ray Treatments in TwoPlants (Basel, Switzerland) · 2020Article
- Floral Induction and Flower Development of Orchids.Frontiers in plant science · 2019Review
- Efficient and heritable transformation of Phalaenopsis orchids.Botanical studies · 2016Article
- Agrobacterium-mediated transformation of the recalcitrant Vanda Kasem's Delight orchid with higher efficiency.TheScientificWorldJournal · 2014Article
- Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement.Frontiers in plant science · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically transformed plants of Cymbidium were regenerated after cocultivating protocorm-like bodies (PLB) with Agrobacterium tumefaciens strain EHA101 (pIG121Hm) that harbored genes for beta-glucuronidase (gus), hygromycin phosphotransferase (hpt) and neomycin phosphotransferase II (nptII). PLB of three genotypes maintained in liquid new Dogashima medium (NDM), were subjected to transformation experiments. The PLB inoculated with Agrobacterium produced secondary PLB, 4 weeks after transfer onto 2.5 g L(-1) gellan gum-solidified NDM containing 10 g L(-1) sucrose, 20 mg L(-1) hygromycin and 40 mg L(-1) meropenem. Transformation efficiency was affected by genotype and the presence of acetosyringone during cocultivation. The highest transformation efficiency was obtained when PLB from the genotype L4 were infected and cocultivated with Agrobacterium on medium containing 100 muM acetosyringone. Transformation of the hygromycin-resistant plantlets regenerated from different sites of inoculated PLB was confirmed by histochemical GUS assay, PCR analysis and Southern blot hybridization.
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Registered trials
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