ArticlePloS one2006
Rho kinase's role in myosin recruitment to the equatorial cortex of mitotic Drosophila S2 cells is for myosin regulatory light chain phosphorylation.
Article in PloS one, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 64 citations in OpenAlex.
- Curving Actin Across Systems: Do Axonal Actin Rings Share Common Underlying Mechanisms?Journal of neurochemistry · 2026Review
- The regulation of actin dynamics during cell division and malignancy.American journal of cancer research · 2021Review
- TheMolecular biology of the cell · 2020Article
- Animal Cell Cytokinesis: The Rho-Dependent Actomyosin-Anilloseptin Contractile Ring as a Membrane Microdomain Gathering, Compressing, and Sorting Machine.Frontiers in cell and developmental biology · 2020Article
- Rho-dependent control of the Citron kinase, Sticky, drives midbody ring maturation.Molecular biology of the cell · 2019Article
- Phosphorylation of the regulatory light chain of myosin in striated muscle: methodological perspectives.European biophysics journal : EBJ · 2016Review
- Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.Molecular biology of the cell · 2016Article
- Cross-regulation between Aurora B and Citron kinase controls midbody architecture in cytokinesis.Open biology · 2016Article
- Phosphorylation of myosin II regulatory light chain by ZIP kinase is responsible for cleavage furrow ingression during cell division in mammalian cultured cells.Biochemical and biophysical research communications · 2015Article
- Two-pore channel 1 interacts with citron kinase, regulating completion of cytokinesis.Channels (Austin, Tex.) · 2015Article
- Genetic suppression of a phosphomimic myosin II identifies system-level factors that promote myosin II cleavage furrow accumulation.Molecular biology of the cell · 2014Article
- Spatiotemporal control of epithelial remodeling by regulated myosin phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Rho GTPases as regulators of mitosis and cytokinesis in mammalian cells.Small GTPases · 2014Review
- Opposing actions of septins and Sticky on Anillin promote the transition from contractile to midbody ring.The Journal of cell biology · 2013Article
- Multiparametric analysis of CLASP-interacting protein functions during interphase microtubule dynamics.Molecular and cellular biology · 2013Article
- Nonmuscle myosin-2: mix and match.Cellular and molecular life sciences : CMLS · 2013Review
- Par-1 controls myosin-II activity through myosin phosphatase to regulate border cell migration.Current biology : CB · 2012Article
- Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis.The Journal of cell biology · 2011Article
- Citron kinase controls abscission through RhoA and anillin.Molecular biology of the cell · 2011Article
- Cytokinesis through biochemical-mechanical feedback loops.Seminars in cell & developmental biology · 2010Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMyosin II recruitment to the equatorial cortex is one of the earliest events in establishment of the cytokinetic contractile ring. In Drosophila S2 cells, we previously showed that myosin II is recruited to the furrow independently of F-actin, and that Rho1 and Rok are essential for this recruitment [1]. Rok phosphorylates several cellular proteins, including the myosin regulatory light chain (RLC). METHODOLOGY/PRINCIPAL
findingsHere we express phosphorylation state mimic constructs of the RLC in S2 cells to examine the role of RLC phosphorylation involving Rok in the localization of myosin. Phosphorylation of the RLC is required for myosin localization to the equatorial cortex during mitosis, and the essential role of Rok in this localization and for cytokinesis is to maintain phosphorylation of the RLC. The ability to regulate the RLC phosphorylation state spatio-temporally is not essential for the myosin localization. Furthermore, the essential role of Citron in cytokinesis is not phosphorylation of the RLC. CONCLUSIONS/SIGNIFICANCE: We conclude that the Rho1 pathway leading to myosin localization to the future cytokinetic furrow is relatively straightforward, where only Rok is needed, and it is only needed to maintain phosphorylation of the myosin RLC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.