ArticleThe Journal of biological chemistry2007
Convergence of p53 and transforming growth factor beta (TGFbeta) signaling on activating expression of the tumor suppressor gene maspin in mammary epithelial cells.
Article in The Journal of biological chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- SERPINB5/TGF-β signaling modulates desmoplakin membrane localization and ameliorates pemphigus vulgaris skin blistering.JCI insight · 2025Article
- Integrating the tumor-suppressive activity of Maspin with p53 in retuning the epithelial homeostasis: A working hypothesis and applicable prospects.Frontiers in oncology · 2022Review
- Her2 promotes early dissemination of breast cancer by suppressing the p38-MK2-Hsp27 pathway that is targetable by Wip1 inhibition.Oncogene · 2020Article
- Upregulation of maspin expression in human cervical carcinoma cells by transforming growth factor β1 through the convergence of Smad and non-Smad signaling pathways.Oncology letters · 2017Article
- Expression patterns of maspin and mutant p53 are associated with the development of gestational trophoblastic neoplasia.Oncology letters · 2016Article
- TGRL Lipolysis Products Induce Stress Protein ATF3 via the TGF-β Receptor Pathway in Human Aortic Endothelial Cells.PloS one · 2015Article
- TGFβ induces "BRCAness" and sensitivity to PARP inhibition in breast cancer by regulating DNA-repair genes.Molecular cancer research : MCR · 2014Article
- Article
- The transcription factor p53: not a repressor, solely an activator.Cell cycle (Georgetown, Tex.) · 2014Article
- Murine microenvironment metaprofiles associate with human cancer etiology and intrinsic subtypes.Clinical cancer research : an official journal of the American Association for Cancer Research · 2013Article
- CCL2 mediates cross-talk between cancer cells and stromal fibroblasts that regulates breast cancer stem cells.Cancer research · 2012Article
- Nuclear translocation of type I transforming growth factor β receptor confers a novel function in RNA processing.Molecular and cellular biology · 2012Article
- TGF-β mediated DNA methylation in prostate cancer.Translational andrology and urology · 2012Article
- Maspin reprograms the gene expression profile of prostate carcinoma cells for differentiation.Genes & cancer · 2011Article
- When tumor suppressor TGFβ meets the HER2 (ERBB2) oncogene.Journal of mammary gland biology and neoplasia · 2011Review
- Identification of phosphorylation sites on extracellular corneal epithelial cell maspin.Proteomics · 2011Article
- Transforming growth factor-β regulates the sphere-initiating stem cell-like feature in breast cancer through miRNA-181 and ATM.Oncogene · 2011Article
- 2D difference gel electrophoresis analysis of different time points during the course of neoplastic transformation of human mammary epithelial cells.Journal of proteome research · 2011Article
- Context-dependent bidirectional regulation of the MutS homolog 2 by transforming growth factor β contributes to chemoresistance in breast cancer cells.Molecular cancer research : MCR · 2010Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Using two-dimensional difference gel electrophoresis, we identified the tumor suppressor gene maspin as a transforming growth factor beta (TGFbeta) target gene in human mammary epithelial cells. TGFbeta up-regulatesMaspin expression both at the RNA and protein levels. This up-regulation required Smad2/3 function and intact p53-binding elements in the Maspin promoter. DNA affinity immunoblot and chromatin immunoprecipitation revealed the presence of both Smads and p53 at the Maspin promoter in TGFbeta-treated cells, suggesting that both transcription factors cooperate to induce Maspin transcription. TGFbeta did not activate Maspin-luciferase reporter in p53-mutant MDA-MB-231 breast cancer cells, which exhibit methylation of the endogenous Maspin promoter. Expression of ectopic p53, however, restored ligand-induced association of Smad2/3 with a transfected Maspin promoter. Stable transfection of Maspin inhibited basal and TGFbeta-stimulated MDA-MB-231 cell motility. Finally, knockdown of endogenous Maspin in p53 wild-type MCF10A/HER2 cells enhanced basal and TGFbeta-stimulated motility. Taken together, these data support cooperation between the p53 and TGFbeta tumor suppressor pathways in the induction of Maspin expression, thus leading to inhibition of cell migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.