ArticleJournal of clinical microbiology2007
Use of the Agilent 2100 bioanalyzer for rapid and reproducible molecular typing of Streptococcus pneumoniae.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Article
- Prevalence and Characteristics of Bacillus cereus Group Isolated from Raw and Pasteurised Milk.Current microbiology · 2020Article
- Article
- Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.Infection and immunity · 2016Article
- Genotyping of Salmonella Typhi using 8-loci multi locus VNTR analysis.Gut pathogens · 2016Article
- A point mutation in cpsE renders Streptococcus pneumoniae nonencapsulated and enhances its growth, adherence and competence.BMC microbiology · 2014Article
- Streptococcus pneumoniae detects and responds to foreign bacterial peptide fragments in its environment.Open biology · 2014Article
- Differentiation of Salmonella strains from the SARA, SARB and SARC reference collections by using three genes PCR-RFLP and the 2100 Agilent Bioanalyzer.Frontiers in microbiology · 2014Article
- 16S rRNA terminal restriction fragment length polymorphism for the characterization of the nasopharyngeal microbiota.PloS one · 2012Article
- A universal method for the identification of bacteria based on general PCR primers.Indian journal of microbiology · 2011Article
- Article
- Molecular epidemiological study of pyrazinamide-resistance in clinical isolates of mycobacterium tuberculosis from South India.International journal of molecular sciences · 2010Article
- Detection of Streptococcus pneumoniae strain cocolonization in the nasopharynx.Journal of clinical microbiology · 2009Article
Corrections and comments
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5 authors.
Funding
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Abstract
Restriction fragment length polymorphism (RFLP) analysis is an economic and fast technique for molecular typing but has the drawback of difficulties in accurately sizing DNA fragments and comparing banding patterns on agarose gels. We aimed to improve RFLP for typing of the important human pathogen Streptococcus pneumoniae and to compare the results with the commonly used typing techniques of pulsed-field gel electrophoresis and multilocus sequence typing. We designed primers to amplify a noncoding region adjacent to the pneumolysin gene. The PCR product was digested separately with six restriction endonucleases, and the DNA fragments were analyzed using an Agilent 2100 bioanalyzer for accurate sizing. The combined RFLP results for all enzymes allowed us to assign each of the 47 clinical isolates of S. pneumoniae tested to one of 33 RFLP types. RFLP analyzed using the bioanalyzer allowed discrimination between strains similar to that obtained by the more commonly used techniques of pulsed-field gel electrophoresis, which discriminated between 34 types, and multilocus sequence typing, which discriminated between 35 types, but more quickly and with less expense. RFLP of a noncoding region using the Agilent 2100 bioanalyzer could be a useful addition to the molecular typing techniques in current use for S. pneumoniae, especially as a first screen of a local population.
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