Evidence map›Paper›PMID 17202218›Full record

ArticleJournal of virology2007

Upstream AP1- and CREB-binding sites confer high basal activity on the adeno-associated virus type 5 capsid gene promoter.

Chaoyang Ye, David J Pintel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Chaoyang YeUniversity of Missouri Medical School, 471f Life Sciences Building, 1201 E. Rollins Road, Columbia, MO 65211-7310, USA.
David J Pintel
University of Missouri · US

Funding

Parvovirus RNA Processing and Gene Expression StrategiesR01AI046458 · NIAID · UNIVERSITY OF MISSOURI-COLUMBIA · PI PINTEL, DAVID J. · 2000 to 2018
$4.6M
Adeno-associated Virus RNA Splicing and PolyadenylationR01AI056310 · NIAID · UNIVERSITY OF MISSOURI-COLUMBIA · PI PINTEL, DAVID J. · 2004 to 2013
$3.4M
NIAID NIH HHS R01 AI046458NIAID NIH HHS R01 AI056310NIAID NIH HHS R01 AI 46458NIAID NIH HHS R01 AI 56310
6 · The paper itself

Abstract

In contrast to the prototype adeno-associated virus type 2 (AAV2), the capsid gene P41 promoter of AAV5, within viral constructs that lack inverted terminal repeat sequences, displays a high basal level of expression in 293 cells in the absence of coinfecting adenovirus. Here we demonstrate that this was due to differences in the relative strengths of the core promoter elements and to the presence of active binding sites for the transcription factors CREB and AP1 within the upstream region of P41 that are absent from the AAV2 capsid gene promoter P40. These differences also governed the relative basal activity of the AAV capsid gene promoters within near-full-length viral genomes.

Indexed as

Promoter Regions, GeneticBase SequenceBinding SitesCapsidCell LineCyclic AMP Response Element-Binding ProteinDependovirusGenes, ReporterHeLa CellsHumansLuciferasesMolecular Sequence DataPlasmidsSequence Homology, Nucleic AcidTranscription Factor AP-1Cyclic AMP Response Element-Binding ProteinLuciferasesTranscription Factor AP-1

Identifiers

PMID17202218
PMCPMC1865965
OpenAlexW2161552297

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.