Evidence map›Paper›PMID 17202216›Full record

ArticleJournal of virology2007

Initiation at the third in-frame AUG codon of open reading frame 3 of the hepatitis E virus is essential for viral infectivity in vivo.

Y W Huang, T Opriessnig, P G Halbur, X J Meng

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 156 citations in OpenAlex.

  1. The prion-like characteristic of ORF3 contributes to virion release and pathogenesis of hepatitis E virus.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  2. mSphere · 2025
    Review
  3. Article
  4. Article
  5. Review
  6. Hepatitis E Virus.Advances in experimental medicine and biology · 2023
    Article
  7. Characteristics and Functions of HEV Proteins.Advances in experimental medicine and biology · 2023
    Article
  8. [Consensus on prevention and treatment of hepatitis E].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2022
    Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Review
  14. Review
  15. Structural and molecular biology of hepatitis E virus.Computational and structural biotechnology journal · 2021
    Review
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article

23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Y W HuangCenter for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, 1410 Price's Fork Road, Blacksburg, VA 24601, USA.
T Opriessnig
P G Halbur
X J Meng
Iowa State University · USVirginia Tech · US

Funding

A Chicken Model to Study Hepatitis E Virus PathogenesisR01AI050611 · NIAID · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI MENG, XIANG-JIN · 2002 to 2023
$6.1M
Hepatitis E Virus Replication and PathogenesisR56AI065546 · NIAID · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI MENG, XIANG-JIN · 2006 to 2006
$336k
NIAID NIH HHS AI 065546NIAID NIH HHS AI 50611NIAID NIH HHS R01 AI050611NIAID NIH HHS R56 AI065546
6 · The paper itself

Abstract

To determine the initiation strategy of the hepatitis E virus (HEV) open reading frame 3 (ORF3), we constructed five HEV mutants with desired mutations in the ORF1 and ORF2 junction region and tested their levels of in vivo infectivity in pigs. A mutant with a C-terminally truncated ORF3 is noninfectious in pigs, indicating that an intact ORF3 is required for in vivo infectivity. Mutations with substitutions in the first in-frame AUG in the junction region or with the same T insertion at the corresponding position of HEV genotype 4 did not affect the virus infectivity or rescue, although mutations with combinations of the two affected virus recovery efficiency, and a single mutation at the third in-frame AUG completely abolished virus infectivity in vivo, indicating that the third in-frame AUG in the junction region is required for virus infection and is likely the authentic initiation site for ORF3. A conserved double stem-loop RNA structure, which may be important for HEV replication, was identified in the junction region. This represents the first report of using a unique homologous pig model system to study the molecular mechanism of HEV replication and to systematically and definitively identify the authentic ORF3 initiation site.

Indexed as

CodonOpen Reading FramesAnimalsBase SequenceHepatitis E virusMolecular Sequence DataMutationNucleic Acid ConformationRNA, ViralSequence Homology, Nucleic AcidSwineTranscription Initiation SiteVirulenceCodonRNA, Viral

Identifiers

PMID17202216
PMCPMC1866010
OpenAlexW2118253663

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.