ArticleJournal of virology2007
Efficient DNA packaging of bacteriophage PRD1 requires the unique vertex protein P6.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Assembly and Evolution of Poxviruses.Advances in experimental medicine and biology · 2024Review
- Half a Century of Research on Membrane-Containing Bacteriophages: Bringing New Concepts to Modern Virology.Viruses · 2019Review
- A structural model of the genome packaging process in a membrane-containing double stranded DNA virus.PLoS biology · 2014Article
- Article
- Identification and characterization of a DNA binding domain on the adenovirus IVa2 protein.Virology · 2012Article
- Condensed genome structure.Advances in experimental medicine and biology · 2012Review
- Review
- Bacteriophage assembly.Viruses · 2011Review
- In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography.Structure (London, England : 1993) · 2010Article
- An icosahedral algal virus has a complex unique vertex decorated by a spike.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Ternary complex formation on the adenovirus packaging sequence by the IVa2 and L4 22-kilodalton proteins.Journal of virology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of bacteriophage PRD1 proceeds via formation of empty procapsids containing an internal lipid membrane, into which the linear double-stranded DNA genome is subsequently packaged. The packaging ATPase P9 and other putative packaging proteins have been shown to be located at a unique vertex of the PRD1 capsid. Here, we describe the isolation and characterization of a suppressor-sensitive PRD1 mutant deficient in the unique vertex protein P6. Protein P6 was found to be an essential part of the PRD1 packaging machinery; its absence leads to greatly reduced packaging efficiency. Lack of P6 was not found to affect particle assembly, because in the P6-deficient mutant infection, wild-type (wt) amounts of particles were produced, although most were empty. P6 was determined not to be a specificity factor, as the few filled particles seen in the P6-deficient infection contained only PRD1-specific DNA. The presence of P6 was not necessary for retention of DNA in the capsid once packaging had occurred, and P6-deficient DNA-containing particles were found to be stable and infectious, albeit not as infectious as wt PRD1 virions. A packaging model for bacteriophage PRD1, based on previous results and those obtained in this study, is presented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.