ArticleThe Journal of cell biology1991
Discrete nuclear domains of poly(A) RNA and their relationship to the functional organization of the nucleus.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers.
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Who cites it
130 citing papers in PubMed, 303 citations in OpenAlex.
- Acidic transcription factors position the genome at nuclear speckles through transcription-dependent and -independent mechanisms.Molecular cell · 2026Article
- Nuclear speckle-associated perispeckle networks partition active chromatin into gene expression niches.Cell reports · 2026Article
- Chromatin-associated intronic RNAs from long genes form introsomes that shape nuclear architecture in neuronal cells.bioRxiv : the preprint server for biology · 2026Article
- Igh nuclear speckle association promotes V(D)J recombination.Nature communications · 2026Article
- RNA localization to nuclear speckles follows splicing logic.Nucleic acids research · 2026Article
- Biomolecular condensates in immune cell fate.Nature reviews. Immunology · 2025Review
- Profiling transcriptome composition and dynamics within nuclear compartments using SLAM-RT&Tag.Molecular cell · 2025Article
- Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architecture.Nucleus (Austin, Tex.) · 2024Article
- Nuclear speckleopathies: developmental disorders caused by variants in genes encoding nuclear speckle proteins.Human genetics · 2024Review
- Evaluating the role of the nuclear microenvironment in gene function by population-based modeling.Nature structural & molecular biology · 2023Article
- Nuclear speckles - a driving force in gene expression.Journal of cell science · 2022Review
- Article
- SAF-A mutants disrupt chromatin structure through dominant negative effects on RNAs associated with chromatin.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Article
- Multiple pathways of toxicity induced byeLife · 2021Article
- Long Noncoding RNAs-Crucial Players Organizing the Landscape of the Neuronal Nucleus.International journal of molecular sciences · 2021Review
- Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins.The Journal of cell biology · 2020Article
- Article
- Nuclear hubs built on RNAs and clustered organization of the genome.Current opinion in cell biology · 2020Review
- Arginine-Enriched Mixed-Charge Domains Provide Cohesion for Nuclear Speckle Condensation.Molecular cell · 2020Article
- NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.Genes & development · 2019Article
70 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The functional organization of the nucleus was studied using a fluorescence microscopy approach which allowed integration of positional information for RNA, DNA, and proteins. In cells from sea urchin to human, nuclear poly(A) RNA was found concentrated primarily within several discrete "transcript domains" which often surrounded nucleoli. Concentrations of poly(A) RNA were coincident with snRNP antigen clusters, providing evidence for the localization of pre-mRNA splicing at these sites. The spatial relationship of transcript domains with respect to various classes of DNA was established, in that the poly(A) RNA-rich regions coincided with discrete regions of low DNA density and were non-randomly distributed with respect to specific DNA sequences. Centromeric DNA and late-replicating DNA did not overlap transcript domains, whereas a subset of early-replicating DNA may. Results indicate that transcript domains do not result directly from a simple clustering of chromatin corresponding to metaphase chromosomes bands. Finally, observations on the reassembly of these domains after mitosis suggest that the clustering of snRNP antigens may be dependent on the reappearance of pol II transcription. Implications of these findings for overall nuclear structure and function are considered, including a discussion of whether transcript domains may be sites of polymerase II transcription reflecting a clustering of active genes.
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