ArticleBMC biotechnology2007
Internalization of novel non-viral vector TAT-streptavidin into human cells.
Article in BMC biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 191 citations in OpenAlex.
- Polymers of functionalized diaminopropionic acid are efficient mediators of active exogenous enzyme delivery into cells.Scientific reports · 2024Article
- Cell-Penetrating Peptides and Transportan.Pharmaceutics · 2021Review
- Tissue-Specific Delivery of CRISPR Therapeutics: Strategies and Mechanisms of Non-Viral Vectors.International journal of molecular sciences · 2020Review
- A platform for discovery of functional cell-penetrating peptides for efficient multi-cargo intracellular delivery.Scientific reports · 2018Article
- Targeted Intracellular Delivery of Antibodies: The State of the Art.Frontiers in pharmacology · 2018Review
- Investigating Engineered Ribonucleoprotein Particles to Improve Oral RNAi Delivery in Crop Insect Pests.Frontiers in physiology · 2017Article
- Determination of cell uptake pathways for tumor inhibitor lysyl oxidase propeptide.Molecular oncology · 2016Article
- Smart hybrid materials by conjugation of responsive polymers to biomacromolecules.Nature materials · 2015Review
- Molecular mechanisms in the dramatic enhancement of HIV-1 Tat transduction by cationic liposomes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Article
- Simulation of fusion-mediated nanoemulsion interactions with model lipid bilayers.Soft matter · 2012Article
- Dynamics of native β-actin mRNA transport in the cytoplasm.Traffic (Copenhagen, Denmark) · 2011Article
- Single-particle tracking as a quantitative microscopy-based approach to unravel cell entry mechanisms of viruses and pharmaceutical nanoparticles.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Efficient Intracellular Delivery of a Pro-Apoptotic Peptide With A pH-Responsive Carrier.Reactive & functional polymers · 2011Article
- Internalization of biotinylated compounds into cancer cells is promoted by a molecular Trojan horse based upon core streptavidin and clostridial C2 toxin.Naunyn-Schmiedeberg's archives of pharmacology · 2011Article
- Multiplexed supramolecular self-assembly for non-viral gene delivery.Biomaterials · 2010Article
- Unveiling the mechanism of uptake and sub-cellular distribution of cerium oxide nanoparticles.Molecular bioSystems · 2010Article
- Transtactin: a universal transmembrane delivery system for Strep-tag II-fused cargos.Journal of cellular and molecular medicine · 2010Article
- Delivery of macromolecules using arginine-rich cell-penetrating peptides: ways to overcome endosomal entrapment.The AAPS journal · 2009Review
- Direct peptide interaction with surface glycosaminoglycans contributes to the cell penetration of maurocalcine.The Journal of biological chemistry · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 4 countries.
Funding
Abstract
backgroundThe cell-penetrating peptide derived from the Human immunodeficiency virus-1 transactivator protein Tat possesses the capacity to promote the effective uptake of various cargo molecules across the plasma membrane in vitro and in vivo. The objective of this study was to characterize the uptake and delivery mechanisms of a novel streptavidin fusion construct, TAT47-57-streptavidin (TAT-SA, 60 kD). SA represents a potentially useful TAT-fusion partner due to its ability to perform as a versatile intracellular delivery vector for a wide array of biotinylated molecules or cargoes.
resultsBy confocal and immunoelectron microscopy the majority of internalized TAT-SA was shown to accumulate in perinuclear vesicles in both cancer and non-cancer cell lines. The uptake studies in living cells with various fluorescent endocytic markers and inhibiting agents suggested that TAT-SA is internalized into cells efficiently, using both clathrin-mediated endocytosis and lipid-raft-mediated macropinocytosis. When endosomal release of TAT-SA was enhanced through the incorporation of a biotinylated, pH-responsive polymer poly(propylacrylic acid) (PPAA), nuclear localization of TAT-SA and TAT-SA bound to biotin was markedly improved. Additionally, no significant cytotoxicity was detected in the TAT-SA constructs.
conclusionThis study demonstrates that TAT-SA-PPAA is a potential non-viral vector to be utilized in protein therapeutics to deliver biotinylated molecules both into cytoplasm and nucleus of human cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.