ArticleJournal of molecular biology2007
Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Extraribosomal Functions of Bacterial Ribosomal Proteins-An Update, 2023.International journal of molecular sciences · 2024Review
- The P-Site Loop of the Universally Conserved Bacterial Ribosomal Protein L5 Is Required for Maintaining Both Translation Rate and Fidelity.International journal of molecular sciences · 2023Article
- Adaptive Responses ofAntimicrobial agents and chemotherapy · 2022Article
- The nuclear-encoded plastid ribosomal protein L18s are essential for plant development.Frontiers in plant science · 2022Article
- Simplification of Ribosomes in Bacteria with Tiny Genomes.Molecular biology and evolution · 2021Article
- Scaffold protein GhMORG1 enhances the resistance of cotton to Fusarium oxysporum by facilitating the MKK6-MPK4 cascade.Plant biotechnology journal · 2020Article
- Article
- Highly Reduced Genome of the New SpeciesmSphere · 2018Article
- Fluorescence bimolecular complementation enables facile detection of ribosome assembly defects in Escherichia coli.RNA biology · 2016Article
- 5SRNAdb: an information resource for 5S ribosomal RNAs.Nucleic acids research · 2016Article
- Regulation of the rplY gene encoding 5S rRNA binding protein L25 in Escherichia coli and related bacteria.RNA (New York, N.Y.) · 2015Article
- Studying the properties of domain I of the ribosomal protein l1: incorporation into ribosome and regulation of the l1 operon expression.The protein journal · 2015Article
- Ribosome biogenesis in african trypanosomes requires conserved and trypanosome-specific factors.Eukaryotic cell · 2014Article
- Protein L5 is crucial for in vivo assembly of the bacterial 50S ribosomal subunit central protuberance.Nucleic acids research · 2012Article
- A novel association between two trypanosome-specific factors and the conserved L5-5S rRNA complex.PloS one · 2012Article
- Structures of domains I and IV from YbbR are representative of a widely distributed protein family.Protein science : a publication of the Protein Society · 2011Article
- Proteomic analysis of Salmonella enterica serovar Enteritidis following propionate adaptation.BMC microbiology · 2010Article
- Inhibition of bacterial ribosome assembly: a suitable drug target?Microbiology and molecular biology reviews : MMBR · 2009Review
- Ribosome biogenesis and the translation process in Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2007Review
- Transcription antitermination by translation initiation factor IF1.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
A specific complex of 5 S rRNA and several ribosomal proteins is an integral part of ribosomes in all living organisms. Here we studied the importance of Escherichia coli genes rplE, rplR and rplY, encoding 5 S rRNA-binding ribosomal proteins L5, L18 and L25, respectively, for cell growth, viability and translation. Using recombineering to create gene replacements in the E. coli chromosome, it was shown that rplE and rplR are essential for cell viability, whereas cells deleted for rplY are viable, but grow noticeably slower than the parental strain. The slow growth of these L25-defective cells can be stimulated by a plasmid expressing the rplY gene and also by a plasmid bearing the gene for homologous to L25 general stress protein CTC from Bacillus subtilis. The rplY mutant ribosomes are physically normal and contain all ribosomal proteins except L25. The ribosomes from L25-defective and parental cells translate in vitro at the same rate either poly(U) or natural mRNA. The difference observed was that the mutant ribosomes synthesized less natural polypeptide, compared to wild-type ribosomes both in vivo and in vitro. We speculate that the defect is at the ribosome recycling step.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.