ArticleArchives of biochemistry and biophysics2007
Escherichia coli as a platform for functional expression of plant P450 carotene hydroxylases.
Article in Archives of biochemistry and biophysics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 65 citations in OpenAlex.
- Genome-Wide Characterization and Expression Analysis ofBiology · 2025Article
- Carotene hydroxylase DcCYP97A3 affects carotenoid metabolic flow and taproot color by influencing the conversion of α-carotene to lutein in carrot.Horticulture research · 2025Article
- Cytochrome P450 CitCYP97B modulates carotenoid accumulation diversity by hydroxylating β-cryptoxanthin in Citrus.Plant communications · 2024Article
- Hydroxylases involved in terpenoid biosynthesis: a review.Bioresources and bioprocessing · 2023Review
- Cytochrome P450 Surface Domains Prevent the β-Carotene Monohydroxylase CYP97H1 ofBiomolecules · 2023Article
- Article
- Metalloenzymes involved in carotenoid biosynthesis in plants.Methods in enzymology · 2022Article
- Violaxanthin: natural function and occurrence, biosynthesis, and heterologous production.Applied microbiology and biotechnology · 2021Review
- When Carotenoid Biosynthesis Genes Met Escherichia coli : The Early Days and These Days.Advances in experimental medicine and biology · 2021Article
- Pathway engineering for high-yield production of lutein in Escherichia coli.Synthetic biology (Oxford, England) · 2021Article
- Manipulation of Synechocystis sp. PCC 6803 as a platform for functional identification of genes involved in carotenoid metabolism.Plant biotechnology journal · 2020Article
- Dynamic metabolic solutions to the sessile life style of plants.Natural product reports · 2018Review
- Carotenoid Profiling of a Red SeaweedMarine drugs · 2018Article
- The Silencing of Carotenoid β-Hydroxylases by RNA Interference in Different Maize Genetic Backgrounds Increases the β-Carotene Content of the Endosperm.International journal of molecular sciences · 2017Article
- Expression and functional analysis of citrus carotene hydroxylases: unravelling the xanthophyll biosynthesis in citrus fruits.BMC plant biology · 2016Article
- Article
- Cloning and Functional Characterization of the Maize (Zea mays L.) Carotenoid Epsilon Hydroxylase Gene.PloS one · 2015Article
- Mechanistic aspects of carotenoid biosynthesis.Chemical reviews · 2014Review
- Evolutionary origins, molecular cloning and expression of carotenoid hydroxylases in eukaryotic photosynthetic algae.BMC genomics · 2013Article
- Synergistic interactions between carotene ring hydroxylases drive lutein formation in plant carotenoid biosynthesis.Plant physiology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Carotenoids and their derivatives are essential for growth, development, and signaling in plants and have an added benefit as nutraceuticals in food crops. Despite the importance of the biosynthetic pathway, there remain open questions regarding some of the later enzymes in the pathway. The CYP97 family of P450 enzymes was predicted to function in carotene ring hydroxylation, to convert provitamin A carotenes to non-provitamin A xanthophylls. However, substrate specificity was difficult to investigate directly in plants, which mask enzyme activities by a complex and dynamic metabolic network. To characterize the enzymes more directly, we amplified cDNAs from a model crop, Oryza sativa, and used functional complementation in Escherichia coli to test activity and specificity of members of Clans A and C. This heterologous system will be valuable for further study of enzyme interactions and substrate utilization needed to understand better the role of CYP97 hydroxylases in plant carotenoid biosynthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.