ArticleMolecular immunology2007
Evidence for preferential Ig gene usage and differential TdT and exonuclease activities in human naïve and memory B cells.
Article in Molecular immunology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- De novo antibody identification in human blood from full-length single B cell transcriptomics and matching haplotype-resolved germline assemblies.Genome research · 2025Article
- Decreased percentage of memory B cells is independently associated with increased susceptibility to infection in patients on maintenance hemodialysis.International urology and nephrology · 2018Article
- In-Depth Analysis of Human Neonatal and Adult IgM Antibody Repertoires.Frontiers in immunology · 2018Article
- Ontogeny-based immunogens for the induction of V2-directed HIV broadly neutralizing antibodies.Immunological reviews · 2017Review
- Multiple Antibody Lineages in One Donor Target the Glycan-V3 Supersite of the HIV-1 Envelope Glycoprotein and Display a Preference for Quaternary Binding.Journal of virology · 2016Article
- Human memory B cells.Leukemia · 2016Review
- Large-scale sequence and structural comparisons of human naive and antigen-experienced antibody repertoires.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Clonify: unseeded antibody lineage assignment from next-generation sequencing data.Scientific reports · 2016Article
- Loss of B Cells in Patients with Heterozygous Mutations in IKAROS.The New England journal of medicine · 2016Article
- Low frequency of broadly neutralizing HIV antibodies during chronic infection even in quaternary epitope targeting antibodies containing large numbers of somatic mutations.Molecular immunology · 2016Article
- The V gene repertoires of classical and atypical memory B cells in malaria-susceptible West African children.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Article
- The shape of the lymphocyte receptor repertoire: lessons from the B cell receptor.Frontiers in immunology · 2013Review
- Mouse marginal zone B cells harbor specificities similar to human broadly neutralizing HIV antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- High-throughput antibody sequencing reveals genetic evidence of global regulation of the naïve and memory repertoires that extends across individuals.Genes and immunity · 2012Article
- Frequency and genetic characterization of V(DD)J recombinants in the human peripheral blood antibody repertoire.Immunology · 2012Article
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- Naive antibody gene-segment frequencies are heritable and unaltered by chronic lymphocyte ablation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Pseudovirion particles bearing native HIV envelope trimers facilitate a novel method for generating human neutralizing monoclonal antibodies against HIV.Journal of acquired immune deficiency syndromes (1999) · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Memory B cells and the antibodies they encode are important for protective immunity against infectious pathogens. Characterization of naïve and memory B cell antibody repertoires will elucidate the molecular basis for the generation of antibody diversity in human B cells and the optimization of antibody structures that bind microbial antigens. In this study we aimed to investigate the influence of antigenic selection on the antibody genes of the two CD27+ memory B cell subsets, comparing them with the naïve repertoire in CD27- cells. We analyzed and compared the Ig heavy chain gene transcripts in three recently defined circulating naïve and memory B cell subsets (CD19+IgD+CD27- [naïve], CD19+IgD+CD27+ [un-class-switched memory] or CD19+IgD- CD27+ [class-switched memory]) at the single cell level. We found similar biased patterns of variable, diversity and joining heavy chain gene usages in all three groups of cells. CD19+IgD+CD27+ memory B cells harbored as diverse an antibody gene repertoire as CD19+IgD-CD27+ memory B cells. Interestingly, CD19+IgD+CD27+ memory B cells possessed a lower frequency of somatic mutations, a higher incidence of exonuclease activity at the 3' end of D regions, and a lower frequency of N and P nucleotide additions at both VH-D and D-JH junctions of CDR3 regions compared to CD19+IgD-CD27+ memory B cells. These data suggest distinct functional mechanisms underlying selection of this unique subset of un-class-switched memory B cells.
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Registered trials
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