ArticleMicrovascular research2007
Modeling of oxygen transport across tumor multicellular layers.
Article in Microvascular research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Quercetin exerts an inhibitory effect on cellular bioenergetics of the B164A5 murine melanoma cell line.Molecular and cellular biochemistry · 2018Article
- 3D culture broadly regulates tumor cell hypoxia response and angiogenesis via pro-inflammatory pathways.Biomaterials · 2015Article
- A method for estimating the oxygen consumption rate in multicellular tumour spheroids.Journal of the Royal Society, Interface · 2014Article
- Article
- Otto Warburg's contributions to current concepts of cancer metabolism.Nature reviews. Cancer · 2011Review
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2 authors.
Funding
Abstract
purposeTumor oxygen level plays a major role in the response of tumors to different treatments. The purpose of this study was to develop a method of determining oxygen transport properties in a recently developed 3D model of tumor parenchyma, the multicellular layer (MCL).
methodsOCM-1 human choroidal melanoma cells were grown as 3D MCLs on collagen-coated culture plate inserts. A recessed-cathode oxygen microelectrode was used to measure oxygen tension (PO(2)) profiles across 8 different MCL from the free surface to the insert membrane. The profiles were fitted to four different one-dimensional diffusion models: 1-, 2-, and 3-region models with uniform oxygen consumption (q) in each region and a modified 3-region model with a central region where q=0 and PO(2)=0.
resultsDepending upon the presence of a central region of anoxia, the PO(2) profiles were fitted best by either the two-region model or the modified 3-region model. Consumption of tumor cells near the insert membrane was higher than that of cells close to the free surface (33.1+/-13.6 x 10(-4) vs. 11.8+/-6.7 x 10(-4) mm Hg/mum(2), respectively).
conclusionsThe model is useful for determining oxygenation and consumption in MCL, especially for cell lines that cannot be grown as spheroids. In the future, this model will permit the study of parameters important in tumor oxygenation in vitro.
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