ArticleMolecular biology reports2007
Optimization of wheat co-transformation procedure with gene cassettes resulted in an improvement in transformation frequency.
Article in Molecular biology reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 36 citations in OpenAlex.
- An established protocol for generating transgenic wheat for wheat functional genomicsFrontiers in plant science · 2022Article
- Recent developments and applications of genetic transformation and genome editing technologies in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Genetic Modification for Wheat Improvement: From Transgenesis to Genome Editing.BioMed research international · 2019Review
- Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species.BMC biotechnology · 2018Article
- A biolistic method for high-throughput production of transgenic wheat plants with single gene insertions.BMC plant biology · 2018Article
- A New Barley Stripe Mosaic Virus Allows Large Protein Overexpression for Rapid Function Analysis.Plant physiology · 2018Article
- Generation of marker-free transgenic hexaploid wheat via an Agrobacterium-mediated co-transformation strategy in commercial Chinese wheat varieties.Plant biotechnology journal · 2017Article
- High frequency production of rapeseed transgenic plants via combination of microprojectile bombardment and secondary embryogenesis of microspore-derived embryos.Molecular biology reports · 2011Article
- AtMYB12 gene: a novel visible marker for wheat transformation.Molecular biology reports · 2011Article
- Unprecedented enhancement of transient gene expression from minimal cassettes using a double terminator.Plant cell reports · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic manipulation using gene cassettes was applied to the elite wheat variety EM12 via particle bombardment, which allows an improvement in transformation frequency. We simultaneously transferred to wheat immature embryos with two non-linked genes, gus and bar, on either separate gene cassettes or one plasmid. The linear gene cassettes were excised and purified by restriction digestion of the plasmid, and consisted of promoters, open reading frames and terminators. No difference was observed in GUS transient expression of between gene cassettes and single whole plasmid. However, the stable transformation frequency was significantly increased to 1.1% using gene cassettes, compared with 0.4% when using single plasmid. Procedures of the efficient co-transformation with gene cassettes were developed. Factors influencing on the transformation frequency were also studied in order to optimize the procedure. These were acceleration pressure, target distance, gold particle size, the quantity ratio of gene cassettes and the age of target explants. Based on the transient and stable expression of the gus gene cassettes, optimization of transformation parameters improved the reproducibility of transformation in the elite wheat variety.
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