ArticleAnnals of surgical oncology2007
Methylation and gene silencing of the Ras-related GTPase gene in lung and breast cancers.
Article in Annals of surgical oncology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Identification and Functional Analysis of Ras-Related Associated with Diabetes Gene (International journal of molecular sciences · 2024Article
- The enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of oral squamous cell carcinoma.Cell death & disease · 2024Article
- High-throughput mechanical phenotyping and transcriptomics of single cells.Nature communications · 2024Article
- SETD8 inhibits ferroptosis in pancreatic cancer by inhibiting the expression of RRAD.Cancer cell international · 2023Article
- Friend or Foe: Regulation, Downstream Effectors of RRAD in Cancer.Biomolecules · 2023Review
- Article
- RRAD expression in gastric and colorectal cancer with peritoneal carcinomatosis.Scientific reports · 2019Article
- RRAD suppresses the Warburg effect by downregulating ACTG1 in hepatocellular carcinoma.OncoTargets and therapy · 2019Article
- TUFT1 is expressed in breast cancer and involved in cancer cell proliferation and survival.Oncotarget · 2017Article
- RRAD inhibits aerobic glycolysis, invasion, and migration and is associated with poor prognosis in hepatocellular carcinoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Ras-related associated with diabetes gene acts as a suppressor and inhibits Warburg effect in hepatocellular carcinoma.OncoTargets and therapy · 2016Article
- RRAD inhibits the Warburg effect through negative regulation of the NF-κB signaling.Oncotarget · 2015Article
- Tumor suppressor p53 negatively regulates glycolysis stimulated by hypoxia through its target RRAD.Oncotarget · 2014Article
- Ras-induced epigenetic inactivation of the RRAD (Ras-related associated with diabetes) gene promotes glucose uptake in a human ovarian cancer model.The Journal of biological chemistry · 2014Article
- Gene silencing associated with SWI/SNF complex loss during NSCLC development.Molecular cancer research : MCR · 2014Article
- DNA methylation-associated inactivation of TGFβ-related genes, DRM/Gremlin, RUNX3, and HPP1 in human cancers.British journal of cancer · 2013Article
- Epigenetic aberrant methylation of tumor suppressor genes in small cell lung cancer.Journal of thoracic disease · 2013Article
- Cigarette smoking and DNA methylation.Frontiers in genetics · 2013Article
- TGF-β mediated DNA methylation in prostate cancer.Translational andrology and urology · 2012Article
- Effects of SMYD3 over-expression on cell cycle acceleration and cell proliferation in MDA-MB-231 human breast cancer cells.Medical oncology (Northwood, London, England) · 2011Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundRRAD, a small Ras-related GTPase, is highly expressed in human skeletal muscle, lung, and heart. Although loss of expression of RRAD in breast cancer cells has been reported and it may act as an oncogene, the mechanism of silencing is unknown.
methodsWe examined (1) mRNA expression of RRAD in lung and breast cancer cell lines using RT-PCR and (2) methylation status of lung and breast cancers.
resultsLoss of RRAD expression was found in 14 of 20 (70%) NSCLC cell lines, 11 of 11 (100%) SCLC cell lines, and 8 of 10 (80%) breast cancer cell lines; expression was not affected in normal bronchial and mammary epithelial cells. Treatment of 23 expression-negative cell lines with a demethylating agent restored expression in all cases. We developed a methylation-specific assay from the analysis of bisulfite sequencing of the 5' region of RRAD in expression-negative and positive cell lines, which resulted in good concordance between methylation and expression. Primary lung and breast cancers showed hypermethylation in 89 of 214 (42%) and 39 of 63 (62%) cases, respectively. RRAD hypermethylation correlated with smoking history and poorer prognosis in lung adenocarcinomas.
conclusionsWe conclude that epigenetic silencing of RRAD is a frequent event in lung and breast cancers, and analysis of it may provide novel opportunities for prognosis and therapy of these cancers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.