ArticleApplied and environmental microbiology2007
Integrated real-time PCR for detection and monitoring of Legionella pneumophila in water systems.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Detection performance of PCR for Legionella pneumophila in environmental samples: a systematic review and meta-analysis.Annals of clinical microbiology and antimicrobials · 2022Pooled it
- A Rapid and Sensitive CRISPR-Cas12a for the Detection ofPolish journal of microbiology · 2025Article
- Comparison between Legiolert and real time PCR in the detection of Legionella pneumophila from environmental water samples.PloS one · 2025Article
- Performance evaluation of a low-throughput qPCR-based Legionella assay for utility as an onsite industrial water system monitoring method.Journal of industrial microbiology & biotechnology · 2024Article
- Risk assessment and quantitative measurement along with monitoring ofNew microbes and new infections · 2022Article
- Severe Pneumonia in Neonates Associated withPathogens (Basel, Switzerland) · 2021Review
- Comparison of Updated Methods forInternational journal of environmental research and public health · 2021Article
- Rapid Testing and Interventions to ControlMicroorganisms · 2021Article
- Water Sampling Module for Collecting and ConcentratingBiosensors · 2021Article
- Water as a Source of Antimicrobial Resistance and Healthcare-Associated Infections.Pathogens (Basel, Switzerland) · 2020Review
- Prevalence ofIranian journal of medical sciences · 2018Review
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- PCR method for the rapid detection and discrimination of Legionella spp. based on the amplification of pcs, pmtA, and 16S rRNA genes.Journal of applied genetics · 2016Article
- Fatal legionellosis after water birth, Texas, USA, 2014.Emerging infectious diseases · 2015Article
- Identification and quantitative detection of Legionella spp. in various aquatic environments by real-time PCR assay.Environmental science and pollution research international · 2013Article
- Application of EMA-qPCR as a complementary tool for the detection and monitoring of Legionella in different water systems.World journal of microbiology & biotechnology · 2012Article
- Detection and Quantification of Legionella pneumophila from Water Systems in Kuwait Residential Facilities.Journal of pathogens · 2012Article
- An international trial of quantitative PCR for monitoring Legionella in artificial water systems.Journal of applied microbiology · 2011Article
- Specific real-time PCR for simultaneous detection and identification of Legionella pneumophila serogroup 1 in water and clinical samples.Applied and environmental microbiology · 2011Article
- High diversity and abundance of Legionella spp. in a pristine river and impact of seasonal and anthropogenic effects.Applied and environmental microbiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluated a ready-to-use real-time quantitative Legionella pneumophila PCR assay system by testing 136 hot-water-system samples collected from 55 sites as well as 49 cooling tower samples collected from 20 different sites, in parallel with the standard culture method. The PCR assay was reproducible and suitable for routine quantification of L. pneumophila. An acceptable correlation between PCR and culture results was obtained for sanitary hot-water samples but not for cooling tower samples. We also monitored the same L. pneumophila-contaminated cooling tower for 13 months by analyzing 104 serial samples. The culture and PCR results were extremely variable over time, but the curves were similar. The differences between the PCR and culture results did not change over time and were not affected by regular biocide treatment. This ready-to-use PCR assay for L. pneumophila quantification could permit more timely disinfection of cooling towers.
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Registered trials
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