ArticleThe Journal of biological chemistry2007
Molecular properties of rhodopsin and rod function.
Article in The Journal of biological chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 72 citations in OpenAlex.
- E122Q rhodopsin: pigment microspectrophotometry, photoreceptor light responses, and bleaching adaptation.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Article
- MKK4 and MKK7 control degeneration of retinal ganglion cell somas and axons after glaucoma-relevant injury.Cell death discovery · 2025Article
- Structure of human green cone opsin yields insights into mechanisms underlying the rapid decay of its active, signaling state.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Modulating signalling lifetime to optimise a prototypical animal opsin for optogenetic applications.Pflugers Archiv : European journal of physiology · 2023Article
- Immediate responses to ambient lightiScience · 2023Article
- Ancient whale rhodopsin reconstructs dim-light vision over a major evolutionary transition: Implications for ancestral diving behavior.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- New insights into the molecular mechanism of rhodopsin retinitis pigmentosa from the biochemical and functional characterization of G90V, Y102H and I307N mutations.Cellular and molecular life sciences : CMLS · 2022Article
- Recreated Ancestral Opsin Associated with Marine to Freshwater Croaker Invasion Reveals Kinetic and Spectral Adaptation.Molecular biology and evolution · 2021Article
- Evolutionary Constraint on Visual and Nonvisual Mammalian Opsins.Journal of biological rhythms · 2021Article
- Sensory Transduction in Photoreceptors and Olfactory Sensory Neurons: Common Features and Distinct Characteristics.Frontiers in cellular neuroscience · 2021Review
- Ground squirrel - A cool model for a bright vision.Seminars in cell & developmental biology · 2020Review
- Analysis of Adult Neural Retina Extracellular Vesicle Release, RNA Transport and Proteomic Cargo.Investigative ophthalmology & visual science · 2020Article
- Cul3-Klhl18 ubiquitin ligase modulates rod transducin translocation during light-dark adaptation.The EMBO journal · 2019Article
- Apo-Opsin Exists in Equilibrium Between a Predominant Inactive and a Rare Highly Active State.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Article
- Evolution of nonspectral rhodopsin function at high altitudes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- In vivo optophysiology reveals that G-protein activation triggers osmotic swelling and increased light scattering of rod photoreceptors.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Spontaneous activation of visual pigments in relation to openness/closedness of chromophore-binding pocket.eLife · 2017Article
- A simplified microwave-based motion detector for home cage activity monitoring in mice.Journal of biological engineering · 2017Article
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Authors and funding
14 authors at 8 institutions in 2 countries.
Funding
Abstract
Signal transduction in rod cells begins with photon absorption by rhodopsin and leads to the generation of an electrical response. The response profile is determined by the molecular properties of the phototransduction components. To examine how the molecular properties of rhodopsin correlate with the rod-response profile, we have generated a knock-in mouse with rhodopsin replaced by its E122Q mutant, which exhibits properties different from those of wild-type (WT) rhodopsin. Knock-in mouse rods with E122Q rhodopsin exhibited a photosensitivity about 70% of WT. Correspondingly, their single-photon response had an amplitude about 80% of WT, and a rate of decline from peak about 1.3 times of WT. The overall 30% lower photosensitivity of mutant rods can be explained by a lower pigment photosensitivity (0.9) and the smaller single-photon response (0.8). The slower decline of the response, however, did not correlate with the 10-fold shorter lifetime of the meta-II state of E122Q rhodopsin. This shorter lifetime became evident in the recovery phase of rod cells only when arrestin was absent. Simulation analysis of the photoresponse profile indicated that the slower decline and the smaller amplitude of the single-photon response can both be explained by the shift in the meta-I/meta-II equilibrium of E122Q rhodopsin toward meta-I. The difference in meta-III lifetime between WT and E122Q mutant became obvious in the recovery phase of the dark current after moderate photobleaching of rod cells. Thus, the present study clearly reveals how the molecular properties of rhodopsin affect the amplitude, shape, and kinetics of the rod response.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.