ArticlePLoS biology2007
Alternative 3' pre-mRNA processing in Saccharomyces cerevisiae is modulated by Nab4/Hrp1 in vivo.
Article in PLoS biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- Nonsense-mediated mRNA decay and metal ion homeostasis and detoxification in Saccharomyces cerevisiae.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2022Review
- Activation and inhibition of nonsense-mediated mRNA decay control the abundance of alternative polyadenylation products.Nucleic acids research · 2020Article
- Review
- Comparative analysis of alternative polyadenylation inGenome research · 2017Article
- Promoter-Terminator Gene Loops Affect Alternative 3'-End Processing in Yeast.The Journal of biological chemistry · 2016Article
- Integrative genome-wide analysis reveals HLP1, a novel RNA-binding protein, regulates plant flowering by targeting alternative polyadenylation.Cell research · 2015Article
- Multilayer regulatory mechanisms control cleavage factor I proteins in filamentous fungi.Nucleic acids research · 2015Article
- Regulation of natural mRNAs by the nonsense-mediated mRNA decay pathway.Eukaryotic cell · 2014Review
- The regulatory role of Pcf11-similar-4 (PCFS4) in Arabidopsis development by genome-wide physical interactions with target loci.BMC genomics · 2013Article
- Post-transcriptional regulation of iron homeostasis in Saccharomyces cerevisiae.International journal of molecular sciences · 2013Review
- Species-specific factors mediate extensive heterogeneity of mRNA 3' ends in yeasts.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- UV damage regulates alternative polyadenylation of the RPB2 gene in yeast.Nucleic acids research · 2013Article
- Structural and biochemical analysis of the assembly and function of the yeast pre-mRNA 3' end processing complex CF I.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Fungal virulence and development is regulated by alternative pre-mRNA 3'end processing in Magnaporthe oryzae.PLoS pathogens · 2011Article
- Yeast pheromone receptor genes STE2 and STE3 are differently regulated at the transcription and polyadenylation level.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The export factor Yra1 modulates mRNA 3' end processing.Nature structural & molecular biology · 2011Article
- Novel protein-protein contacts facilitate mRNA 3'-processing signal recognition by Rna15 and Hrp1.Journal of molecular biology · 2010Article
- Molecular mechanisms of eukaryotic pre-mRNA 3' end processing regulation.Nucleic acids research · 2010Review
- Long 3'-UTRs target wild-type mRNAs for nonsense-mediated mRNA decay in Saccharomyces cerevisiae.Nucleic acids research · 2009Article
- COMIT: identification of noncoding motifs under selection in coding sequences.Genome biology · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The Saccharomyces cerevisiae RNA-binding protein Nab4/Hrp1 is a component of the cleavage factor complex required for 3' pre-mRNA processing. Although the precise role of Nab4/Hrp1 remains unclear, it has been implicated in correct positioning of the cleavage site in vitro. Here, we show that mutation or overexpression of NAB4/HRP1 alters polyA cleavage site selection in vivo. Using bioinformatic analysis, we identified four related motifs that are statistically enriched in Nab4-associated transcripts; each motif is similar to the known binding site for Nab4/Hrp1. Site-directed mutations in predicted Nab4/Hrp1 binding elements result in decreased use of adjacent cleavage sites. Additionally, we show that the nab4-7 mutant displays a striking resistance to toxicity from excess copper. We identify a novel target of alternative 3' pre-mRNA processing, CTR2, and demonstrate that CTR2 is required for the copper resistance phenotype in the nab4-7 strain. We propose that alternative 3' pre-mRNA processing is mediated by a Nab4-based mechanism and that these alternative processing events could help control gene expression as part of a physiological response in S. cerevisiae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.