ArticleMolecular biology of the cell2007
Asymmetric localization of calpain 2 during neutrophil chemotaxis.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
- A Putative Role for TRPC6 in Immune-Mediated Kidney Injury.International journal of molecular sciences · 2023Review
- Abnormal neutrophil polarization in chronic obstructive pulmonary disease and how cigarette smoke extracts attract neutrophils.Annals of translational medicine · 2022Article
- Genome-Wide Association of New-Onset Hypertension According to Renin Concentration: The Korean Genome and Epidemiology Cohort Study.Journal of cardiovascular development and disease · 2022Article
- Clathrin-independent endocytosis, retrograde trafficking, and cell polarity.Current opinion in cell biology · 2020Review
- Calpain2 mediates Rab5-driven focal adhesion disassembly and cell migration.Cell adhesion & migration · 2018Article
- Specific calpain inhibition protects kidney against inflammaging.Scientific reports · 2017Article
- Genome-wide analysis of differential transcriptional and epigenetic variability across human immune cell types.Genome biology · 2017Article
- Chemotactic G protein-coupled receptors control cell migration by repressing autophagosome biogenesis.Autophagy · 2016Article
- Ezrin regulates focal adhesion and invadopodia dynamics by altering calpain activity to promote breast cancer cell invasion.Molecular biology of the cell · 2015Article
- Mammalian target of rapamycin and Rictor control neutrophil chemotaxis by regulating Rac/Cdc42 activity and the actin cytoskeleton.Molecular biology of the cell · 2013Article
- Calpain-dependent cytoskeletal rearrangement exploited for anthrax toxin endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Proteomic identification of VEGF-dependent protein enrichment to membrane caveolar-raft microdomains in endothelial progenitor cells.Molecular & cellular proteomics : MCP · 2013Article
- Fibroblast Migration Is Regulated by Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) Protein.PloS one · 2013Article
- CalpB modulates border cell migration in Drosophila egg chambers.BMC developmental biology · 2012Article
- The non-receptor tyrosine kinase Lyn controls neutrophil adhesion by recruiting the CrkL-C3G complex and activating Rap1 at the leading edge.Journal of cell science · 2011Article
- An obligate role for membrane-associated neutral sphingomyelinase activity in orienting chemotactic migration of human neutrophils.American journal of respiratory cell and molecular biology · 2011Article
- Calpain 2 is required for glioblastoma cell invasion: regulation of matrix metalloproteinase 2.Neurochemical research · 2010Article
- Calpain inhibition impairs TNF-alpha-mediated neutrophil adhesion, arrest and oxidative burst.Molecular immunology · 2010Article
- The role of calpain-calpastatin system in the development of stress urinary incontinence.International urogynecology journal · 2010Article
- Epac increases melanoma cell migration by a heparan sulfate-related mechanism.American journal of physiology. Cell physiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Chemoattractants induce neutrophil polarization through localized polymerization of F-actin at the leading edge. The suppression of rear and lateral protrusions is required for efficient chemotaxis and involves the temporal and spatial segregation of signaling molecules. We have previously shown that the intracellular calcium-dependent protease calpain is required for cell migration and is involved in regulating neutrophil chemotaxis. Here, we show that primary neutrophils and neutrophil-like HL-60 cells express both calpain 1 and calpain 2 and that chemoattractants induce the asymmetric recruitment of calpain 2, but not calpain 1, to the leading edge of polarized neutrophils and differentiated HL-60 cells. Using time-lapse microscopy, we show that enrichment of calpain 2 at the leading edge occurs during early pseudopod formation and that its localization is sensitive to changes in the chemotactic gradient. We demonstrate that calpain 2 is recruited to lipid rafts and that cholesterol depletion perturbs calpain 2 localization, suggesting that its enrichment at the front requires proper membrane organization. Finally, we show that catalytic activity of calpain is required to limit pseudopod formation in the direction of chemoattractant and for efficient chemotaxis. Together, our findings identify calpain 2 as a novel component of the frontness signal that promotes polarization during chemotaxis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.