ArticleProceedings of the National Academy of Sciences of the United States of America2007
A singular enzymatic megacomplex from Bacillus subtilis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed.
- Subcellular visualization and quantification of cyanotoxin synthesis in cyanobacteria reveals distinct compartmentation.Scientific reports · 2026Article
- Comprehensive mapping of transcription terminator Rho utilization (Rut) sites across the Bacillus subtilis genome.Nucleic acids research · 2025Article
- Inhibition of Surfactin Biosynthesis in Bacillus Subtilis Using Cell-Permeable Adenylation Domain Inhibitors.Chembiochem : a European journal of chemical biology · 2025Article
- Article
- Systematic characterization of horizontally transferred biosynthetic gene clusters in the human gut microbiota using HTBGC-Finder.iMetaOmics · 2025Article
- Gut microbiota dysbiosis and associated immune response in systemic lupus erythematosus: impact of disease and treatment.Gut pathogens · 2025Article
- The Utilization ofMicroorganisms · 2024Article
- More than a colour; how pigment influences colourblind microbes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.Methods (San Diego, Calif.) · 2024Review
- Lipopeptides as rhizosphere public goods for microbial cooperation.Microbiology spectrum · 2024Article
- Substrate specificity of a ketosynthase domain involved in bacillaene biosynthesis.Beilstein journal of organic chemistry · 2024Article
- Whole-genome sequencing and antimicrobial potential of bacteria isolated from Polish honey.Applied microbiology and biotechnology · 2023Article
- Dissection of 3D chromosome organization inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Plant-associated Bacillus mobilizes its secondary metabolites upon perception of the siderophore pyochelin produced by a Pseudomonas competitor.The ISME journal · 2023Article
- Nutrient Availability and Biofilm Polysaccharide Shape the Bacillaene-Dependent Antagonism of Bacillus subtilis against Salmonella Typhimurium.Microbiology spectrum · 2022Article
- The structure of a polyketide synthase bimodule core.Science advances · 2022Article
- Impact ofMicrobiology spectrum · 2022Article
- Engineering the stambomycin modular polyketide synthase yields 37-membered mini-stambomycins.Nature communications · 2022Article
- Resolving the conflict between antibiotic production and rapid growth by recognition of peptidoglycan of susceptible competitors.Nature communications · 2022Article
- Phage tail-like nanostructures affect microbial interactions between Streptomyces and fungi.Scientific reports · 2021Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Nonribosomal peptide synthetases (NRPS), polyketide synthases (PKS), and hybrid NRPS/PKS are of particular interest, because they produce numerous therapeutic agents, have great potential for engineering novel compounds, and are the largest enzymes known. The predicted masses of known enzymatic assembly lines can reach almost 5 megadaltons, dwarfing even the ribosome (approximately 2.6 megadaltons). Despite their uniqueness and importance, little is known about the organization of these enzymes within the native producer cells. Here we report that an 80-kb gene cluster, which occupies approximately 2% of the Bacillus subtilis genome, encodes the subunits of approximately 2.5 megadalton active hybrid NRPS/PKS. Many copies of the NRPS/PKS assemble into a single organelle-like membrane-associated complex of tens to hundreds of megadaltons. Such an enzymatic megacomplex is unprecedented in bacterial subcellular organization and has important implications for engineering novel NRPS/PKSs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.