ArticleMethods (San Diego, Calif.)2007
Analyzing the ATR-mediated checkpoint using Xenopus egg extracts.
Article in Methods (San Diego, Calif.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- The Sm core components of small nuclear ribonucleoproteins promote homologous recombination repair.DNA repair · 2021Article
- DNA Damage Response in Xenopus laevis Cell-Free Extracts.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Reconstituting Nuclear and Chromosome Dynamics UsingCold Spring Harbor protocols · 2019Article
- Protein interactomes of protein phosphatase 2A B55 regulatory subunits reveal B55-mediated regulation of replication protein A under replication stress.Scientific reports · 2018Article
- Dna2 initiates resection at clean DNA double-strand breaks.Nucleic acids research · 2017Article
- Protein phosphatase 1 and phosphatase 1 nuclear targeting subunit-dependent regulation of DNA-dependent protein kinase and non-homologous end joining.Nucleic acids research · 2017Article
- Non-homologous end joining repair in Xenopus egg extract.Scientific reports · 2016Article
- Study of the DNA damage checkpoint using Xenopus egg extracts.Journal of visualized experiments : JoVE · 2012Article
- Greatwall and Polo-like kinase 1 coordinate to promote checkpoint recovery.The Journal of biological chemistry · 2011Article
- A novel role for greatwall kinase in recovery from DNA damage.Cell cycle (Georgetown, Tex.) · 2010Article
- Analysis of histones and chromatin in Xenopus laevis egg and oocyte extracts.Methods (San Diego, Calif.) · 2010Article
- Continued primer synthesis at stalled replication forks contributes to checkpoint activation.The Journal of cell biology · 2010Article
- Repo-man controls a protein phosphatase 1-dependent threshold for DNA damage checkpoint activation.Current biology : CB · 2010Article
- The Fanconi anemia protein FANCM is controlled by FANCD2 and the ATR/ATM pathways.The Journal of biological chemistry · 2009Article
- The role of Dbf4/Drf1-dependent kinase Cdc7 in DNA-damage checkpoint control.Molecular cell · 2008Article
- The Power of Xenopus Egg Extract for Reconstitution of Centromere and Kinetochore Function.Progress in molecular and subcellular biologyReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Our knowledge of cell cycle events such as DNA replication and mitosis has been advanced significantly through the use of Xenopus egg extracts as a model system. More recently, Xenopus extracts have been used to investigate the cellular mechanisms that ensure accurate and complete duplication of the genome, processes otherwise known as the DNA damage and replication checkpoints. Here we describe several Xenopus extract methods that have advanced the study of the ATR-mediated checkpoints. These include a protocol for the preparation of nucleoplasmic extract (NPE), which is a soluble extract system useful for studying nuclear events such as DNA replication and checkpoints. In addition, we describe several key assays for studying checkpoint activation as well as methods for using small DNA structures to activate ATR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.