ArticleApplied and environmental microbiology2007
Transgalactosylation in a water-solvent biphasic reaction system with beta-galactosidase displayed on the surfaces of Bacillus subtilis spores.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 73 citations in OpenAlex.
- Polymer chemistry at the living-material interface.Chemical science · 2026Review
- Degradation of beechwood xylan using food-grade bacteria-like particles displaying β-xylosidase from Limosilactobacillus fermentum.Bioresources and bioprocessing · 2025Article
- Biosynthesis and one-step enrichment process of potentially prebiotic cello-oligosaccharides produced by β-glucosidase from Fusarium solani.Archives of microbiology · 2024Article
- Applications ofMicroorganisms · 2024Review
- Article
- Artificially sporulated Escherichia coli cells as a robust cell factory for interfacial biocatalysis.Nature communications · 2022Article
- Advances in engineeredSynthetic and systems biotechnology · 2021Review
- Orally Administered Bacillus Spores Expressing an Extracellular Vesicle-Derived Tetraspanin Protect Hamsters Against Challenge Infection With Carcinogenic Human Liver Fluke.The Journal of infectious diseases · 2021Article
- Highly sensitive protein detection using recombinant spores and lateral flow immunoassay.Analytical and bioanalytical chemistry · 2021Article
- Constructing an EfficientMolecules (Basel, Switzerland) · 2021Article
- Applications of Bacillus subtilis Spores in Biotechnology and Advanced Materials.Applied and environmental microbiology · 2020Review
- Production of trehalose with trehalose synthase expressed and displayed on the surface of Bacillus subtilis spores.Microbial cell factories · 2019Article
- Surface display of the thermophilic lipase Tm1350 on the spore of Bacillus subtilis by the CotB anchor protein.Extremophiles : life under extreme conditions · 2015Article
- A system of vectors for Bacillus subtilis spore surface display.Microbial cell factories · 2014Article
- Chemoenzymatic synthesis of N-acetyl-D-neuraminic acid from N-acetyl-D-glucosamine by using the spore surface-displayed N-acetyl-D-neuraminic acid aldolase.Applied and environmental microbiology · 2011Article
- Production of N-acetyl-D-neuraminic acid by use of an efficient spore surface display system.Applied and environmental microbiology · 2011Article
- Display of recombinant proteins on Bacillus subtilis spores, using a coat-associated enzyme as the carrier.Applied and environmental microbiology · 2010Article
- Expression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis spores.Microbial cell factories · 2010Article
- Display of proteins on Bacillus subtilis endospores.Cellular and molecular life sciences : CMLS · 2009Review
- CotC-CotU heterodimerization during assembly of the Bacillus subtilis spore coat.Journal of bacteriology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ever-increasing industrial demand for biocatalysis necessitates innovations in the preparation and stabilization of biocatalysts. In this study, we demonstrated that beta-galactosidase (beta-Gal) displayed on Bacillus spores by fusion to the spore coat proteins (CotG) may be used as a whole-cell immobilized biocatalyst for transgalactosylation in water-solvent biphasic reaction systems. The resulting spores had a specific hydrolytic activity of 5 x 10(3) U/g (dry weight) of spores. The beta-Gal was tightly attached to the spore surface and was more stable in the presence of various organic solvents than its native form was. The thermostability of the spore-displayed enzyme was also increased, and the enzyme was further stabilized by chemically cross-linking it with glutaraldehyde. With spore-displayed beta-Gal, octyl-beta-D-galactopyranoside was synthesized at concentrations up to 27.7 mM (8.1 g/liter) with a conversion yield of 27.7% (wt/wt) after 24 h from 100 mM lactose and 100 mM octanol dissolved in phosphate buffer and ethyl ether, respectively. Interestingly, the spores were found to partition mainly at the interface between the water and solvent phases, and they were more available to catalysis between the two phases, as determined by light microscopy and confocal fluorescence microscopy. We propose that spore display not only offers a new and facile way to construct robust biocatalysts but also provides a novel basis for phase transfer biocatalytic processes.
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