ArticleMolecular and cellular biology2007
Two G-rich regulatory elements located adjacent to and 440 nucleotides downstream of the core poly(A) site of the intronless melanocortin receptor 1 gene are critical for efficient 3' end processing.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Unique features of transcription termination and initiation at closely spaced tandem human genes.Molecular systems biology · 2022Article
- G-quadruplex occurrence and conservation: more than just a question of guanine-cytosine content.NAR genomics and bioinformatics · 2022Article
- Biophysical characterisation of the Bcl-x pre-mRNA and binding specificity of the ellipticine derivative GQC-05: Implication for alternative splicing regulation.Frontiers in molecular biosciences · 2022Article
- Advances in the Bioinformatics Knowledge of mRNA Polyadenylation in Baculovirus Genes.Viruses · 2020Review
- G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA.Nucleic acids research · 2020Article
- Intrinsic Regulatory Role of RNA Structural Arrangement in Alternative Splicing Control.International journal of molecular sciences · 2020Review
- Article
- NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation.Theranostics · 2019Article
- Reconstitution of mammalian cleavage factor II involved in 3' processing of mRNA precursors.RNA (New York, N.Y.) · 2018Article
- Targeting the Polyadenylation Signal of Pre-mRNA: A New Gene Silencing Approach for Facioscapulohumeral Dystrophy.International journal of molecular sciences · 2018Review
- RNA G-Quadruplexes in Biology: Principles and Molecular Mechanisms.Journal of molecular biology · 2017Review
- Article
- Subcellular RNA profiling links splicing and nuclear DICER1 to alternative cleavage and polyadenylation.Genome research · 2016Article
- Functional Characterization of MC1R-TUBB3 Intergenic Splice Variants of the Human Melanocortin 1 Receptor.PloS one · 2015Article
- MC1R, the cAMP pathway, and the response to solar UV: extending the horizon beyond pigmentation.Pigment cell & melanoma research · 2014Review
- Poly(A) polymerase (PAP) diversity in gene expression--star-PAP vs canonical PAP.FEBS letters · 2014Review
- Exploring mRNA 3'-UTR G-quadruplexes: evidence of roles in both alternative polyadenylation and mRNA shortening.Nucleic acids research · 2013Article
- Long-distance regulation of Add2 pre-mRNA3'end processing.RNA biology · 2013Article
- Alterations in polyadenylation and its implications for endocrine disease.Frontiers in endocrinology · 2013Article
- Ending the message: poly(A) signals then and now.Genes & development · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cleavage and polyadenylation is an essential processing reaction required for the maturation of pre-mRNAs into stable, export- and translation-competent mature mRNA molecules. This reaction requires the assembly of a multimeric protein complex onto a bipartite core sequence element consisting of an AAUAAA hexamer and a GU/U-rich downstream sequence element. In this study we have analyzed 3' end processing of the human melanocortin 1 receptor gene (MC1R). The MC1R gene is an intron-free transcription unit, and its poly(A) site lacks a defined U/GU-rich element. We describe two G-rich sequence elements that are critical for efficient cleavage at the MC1R poly(A) site. The first element is located 30 nucleotides downstream of the cleavage site and acts as an essential closely positioned enhancer. The second G-rich region is positioned more than 440 nucleotides downstream of the MC1R processing site and is instrumental for optimal processing efficiency. Both G-rich sequences contain clusters of heterogeneous nuclear ribonucleoprotein binding motifs and act together to enhance cleavage at the MC1R poly(A) site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.