ArticleJournal of bacteriology2007
Functional analysis of nine putative chemoreceptor proteins in Sinorhizobium meliloti.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- ThemBio · 2026Article
- Functional insights into the photoactive yellow protein family from homologs, multidomain proteins, and inferredJournal of bacteriology · 2026Review
- Unexpected modulation of Hna phage defense activity by the symbiotic regulator NolR.Journal of bacteriology · 2025Article
- Article
- Azospirillum brasilense AerC and Tlp4b Cytoplasmic Chemoreceptors Are Promiscuous and Interact with the Two Membrane-Bound Chemotaxis Signaling Clusters Mediating Chemotaxis Responses.Journal of bacteriology · 2023Article
- Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions.Methods in microbiology · 2023Article
- A Tar aspartate receptor and Rubisco-like protein substitute biotin in the growth of rhizobial strains.Microbiology (Reading, England) · 2022Article
- McpT, a Broad-Range Carboxylate Chemoreceptor in Sinorhizobium meliloti.Journal of bacteriology · 2021Article
- Chemotaxis of Beneficial Rhizobacteria to Root Exudates: The First Step towards Root-Microbe Rhizosphere Interactions.International journal of molecular sciences · 2021Review
- Migration and accumulation of bacteria with chemotaxis and chemokinesis.The European physical journal. E, Soft matter · 2021Article
- Rhizobial Chemotaxis and Motility Systems at Work in the Soil.Frontiers in plant science · 2021Review
- Rhizobial Chemoattractants, the Taste and Preferences of Legume Symbionts.Frontiers in plant science · 2021Review
- Programmed Proteolysis of Chemotaxis Proteins in Sinorhizobium meliloti: Features in the C-Terminal Region Control McpU Degradation.Journal of bacteriology · 2020Article
- Cellular Stoichiometry of Chemotaxis Proteins inJournal of bacteriology · 2020Article
- Article
- Chemotactic Host-Finding Strategies of Plant Endoparasites and Endophytes.Frontiers in plant science · 2020Review
- An Updated Perspective onFrontiers in microbiology · 2020Article
- Sinorhizobium meliloti Chemoreceptor McpV Senses Short-Chain Carboxylates via Direct Binding.Journal of bacteriology · 2018Article
- Genome-wide identification of genes directly regulated by ChvI and a consensus sequence for ChvI binding in Sinorhizobium meliloti.Molecular microbiology · 2018Article
- Cellular Stoichiometry of Methyl-Accepting Chemotaxis Proteins in Sinorhizobium meliloti.Journal of bacteriology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of the symbiotic soil bacterium Sinorhizobium meliloti contains eight genes coding for methyl-accepting chemotaxis proteins (MCPs) McpS to McpZ and one gene coding for a transducer-like protein, IcpA. Seven of the MCPs are localized in the cytoplasmic membrane via two membrane-spanning regions, whereas McpY and IcpA lack such hydrophobic regions. The periplasmic regions of McpU, McpV, and McpX contain the small-ligand-binding domain Cache. In addition, McpU possesses the ligand-binding domain TarH. By probing gene expression with lacZ fusions, we have identified mcpU and mcpX as being highly expressed. Deletion of any one of the receptor genes caused impairments in the chemotactic response toward most organic acids, amino acids, and sugars in a swarm plate assay. The data imply that chemoreceptor proteins in S. meliloti can sense more than one class of carbon source and suggest that many or all receptors work as an ensemble. Tactic responses were virtually eliminated for a strain lacking all nine receptor genes. Capillary assays revealed three important sensors for the strong attractant proline: McpU, McpX, and McpY. Receptor deletions variously affected free-swimming speed and attractant-induced chemokinesis. Noticeably, cells lacking mcpU were swimming 9% slower than the wild-type control. We infer that McpU inhibits the kinase activity of CheA in the absence of an attractant. Cells lacking one of the two soluble receptors were impaired in chemokinetic proficiency by more than 50%. We propose that the internal sensors, IcpA and the PAS domain containing McpY, monitor the metabolic state of S. meliloti.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.