ArticlePlant physiology2007
Strategies for functional validation of genes involved in reproductive stages of orchids.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 95 citations in OpenAlex.
- R2R3-MYB genes coordinate conical cell development and cuticular wax biosynthesis in Phalaenopsis aphrodite.Plant physiology · 2022Article
- Virus-Induced Gene Silencing in Wheat and Related Monocot Species.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Argonaute 5 family proteins play crucial roles in the defence against Cymbidium mosaic virus and Odontoglossum ringspot virus in Phalaenopsis aphrodite subsp. formosana.Molecular plant pathology · 2021Article
- Genome-wide analysis of small RNAs from Odontoglossum ringspot virus and Cymbidium mosaic virus synergistically infecting Phalaenopsis.Molecular plant pathology · 2020Article
- Spike Activator 1, Encoding a bHLH, Mediates Axillary Bud Development and Spike Initiation inInternational journal of molecular sciences · 2019Article
- Cucumber mosaic virus-induced gene silencing in banana.Scientific reports · 2019Article
- Gene silencing approaches through virus-based vectors: speeding up functional genomics in monocots.Plant molecular biology · 2019Review
- PeERF1, a SHINE-Like Transcription Factor, Is Involved in Nanoridge Development on Lip Epidermis ofFrontiers in plant science · 2019Article
- Dissecting the Function of MADS-Box Transcription Factors in Orchid Reproductive Development.Frontiers in plant science · 2019Review
- PbbHLH4 regulates floral monoterpene biosynthesis in Phalaenopsis orchids.Journal of experimental botany · 2018Article
- An efficient and improved method for virus-induced gene silencing in sorghum.BMC plant biology · 2018Article
- A Dual RepeatFrontiers in plant science · 2018Article
- Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging.Plant methods · 2018Article
- Post genomics era for orchid research.Botanical studies · 2017Review
- Current progress in orchid flowering/flower development research.Plant signaling & behavior · 2017Review
- A Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize.Plant physiology · 2016Article
- Building a Genetic Manipulation Tool Box for Orchid Biology: Identification of Constitutive Promoters and Application of CRISPR/Cas9 in the Orchid,Frontiers in plant science · 2016Article
- Three R2R3-MYB transcription factors regulate distinct floral pigmentation patterning in Phalaenopsis spp.Plant physiology · 2015Article
- Functional Characterization of Phalaenopsis aphrodite Flowering Genes PaFT1 and PaFD.PloS one · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants in the largest family of angiosperms, Orchidaceae, are diverse in both specialized pollination and ecological strategies and provide a rich source for investigating evolutionary relationships and developmental biology. However, studies in orchids have been hindered by several challenges that include low transformation efficiency and long regeneration time. To overcome such obstacles, we selected a symptomless cymbidium mosaic virus (CymMV) isolate for constructing virus-induced gene-silencing vectors. The feasibility of the virus vectors was first assessed with use of an orchid phytoene desaturase gene. The vector was able to induce gene silencing in orchids; however, because of the slow growth of orchids, the commonly used phytoene desaturase gene was not a good visual marker in orchids. We inserted a 150-nucleotide unique region of a B-class MADS-box family gene, PeMADS6, into pCymMV-pro60. The transcription level of PeMADS6 in inoculated Phalaenopsis plants was reduced by up to 73%, but no effect was observed for other MADS-box family genes. In contrast, in Phalaenopsis plants inoculated with CymMV transcripts containing 500 nucleotides of PeMADS6, a conserved region among MADS-box genes, the transcription level of PeMADS6 and the B- and C-class MADS-box genes was reduced by up to 97.8% as compared with plants inoculated with the vector alone. Flower morphology was affected in the MADS-box family gene-silenced plants as well. This in vivo experiment demonstrates an efficient way to study genes involved in the reproductive stage of plants with a long life cycle.
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