ArticleBiophysical journal2007
Protein stability in ice.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 91 citations in OpenAlex.
- Encapsulation of Protein-Based Drug Carriers for Controlled Loading and Releasing Efficiency: A Review.The protein journal · 2026Review
- Impact of single freeze-thaw cycles on human serum proteins: Implications for mass spectrometry biomarker validation.iScience · 2026Article
- Formulation Factors Affecting the Formation of Visible-Bubbles During the Reconstitution Process of Freeze-Dried Etanercept Formulations: Protein Concentration, Stabilizers, and Surfactants.The AAPS journal · 2025Article
- A Roadmap to Cardiac Tissue-Engineered Construct Preservation: Insights from Cells, Tissues, and Organs.Advanced materials (Deerfield Beach, Fla.) · 2021Review
- The Role of Cyclodextrins against Interface-Induced Denaturation in Pharmaceutical Formulations: A Molecular Dynamics Approach.Molecular pharmaceutics · 2021Article
- Machine learning and statistical analyses for extracting and characterizing "fingerprints" of antibody aggregation at container interfaces from flow microscopy images.Biotechnology and bioengineering · 2020Article
- Characterizing and Minimizing Aggregation and Particle Formation of Three Recombinant Fusion-Protein Bulk Antigens for Use in a Candidate Trivalent Rotavirus Vaccine.Journal of pharmaceutical sciences · 2020Article
- A beetle antifreeze protein protects lactate dehydrogenase under freeze-thawing.International journal of biological macromolecules · 2019Article
- Protein/Ice Interaction: High-Resolution Synchrotron X-ray Diffraction Differentiates Pharmaceutical Proteins from Lysozyme.The journal of physical chemistry. B · 2019Article
- Interfacial Stress in the Development of Biologics: Fundamental Understanding, Current Practice, and Future Perspective.The AAPS journal · 2019Article
- Impact of Buffer, Protein Concentration and Sucrose Addition on the Aggregation and Particle Formation during Freezing and Thawing.Pharmaceutical research · 2018Article
- Microheterogeneity in frozen protein solutions.International journal of pharmaceutics · 2015Article
- Protein freeze concentration and micro-segregation analysed in a temperature-controlled freeze container.Biotechnology reports (Amsterdam, Netherlands) · 2015Article
- Freezing-induced perturbation of tertiary structure of a monoclonal antibody.Journal of pharmaceutical sciences · 2014Article
- Structural characterization of IgG1 mAb aggregates and particles generated under various stress conditions.Journal of pharmaceutical sciences · 2014Article
- In situ spectroscopic quantification of protein-ice interactions.The journal of physical chemistry. B · 2013Article
- Protein brownian rotation at the glass transition temperature of a freeze-concentrated buffer probed by superparamagnetic nanoparticles.Biophysical journal · 2013Article
- Beyond glass transitions: studying the highly viscous and elastic behavior of frozen protein formulations using low temperature rheology and its potential implications on protein stability.Pharmaceutical research · 2013Article
- Frozen state storage instability of a monoclonal antibody: aggregation as a consequence of trehalose crystallization and protein unfolding.Pharmaceutical research · 2011Article
- Cavity-creating mutations in Pseudomonas aeruginosa azurin: effects on protein dynamics and stability.Biophysical journal · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study presents an experimental approach, based on the change of Trp fluorescence between native and denatured states of proteins, which permits to monitor unfolding equilibria and the thermodynamic stability (DeltaG degrees ) of these macromolecules in frozen aqueous solutions. The results obtained by guanidinium chloride denaturation of the azurin mutant C112S from Pseudomonas aeruginosa, in the temperature range from -8 to -16 degrees C, demonstrate that the stability of the native fold may be significantly perturbed in ice depending mainly on the size of the liquid water pool (V(L)) in equilibrium with the solid phase. The data establish a threshold, around V(L)=1.5%, below which in ice DeltaG degrees decreases progressively relative to liquid state, up to 3 kcal/mole for V(L)=0.285%. The sharp dependence of DeltaG degrees on V(L) is consistent with a mechanism based on adsorption of the protein to the ice surface. The reduction in DeltaG degrees is accompanied by a corresponding decrease in m-value indicating that protein-ice interactions increase the solvent accessible surface area of the native fold or reduce that of the denatured state, or both. The method opens the possibility for examining in a more quantitative fashion the influence of various experimental conditions on the ice perturbation and in particular to test the effectiveness of numerous additives used in formulations to preserve labile pharmaco proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.