ArticleThe Journal of biological chemistry2007
Methyltransferase that modifies guanine 966 of the 16 S rRNA: functional identification and tertiary structure.
Article in The Journal of biological chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed.
- Compendium of RNA modifications for bacterial stress adaptation.Microbiology and molecular biology reviews : MMBR · 2026Review
- Post-Transcriptional Modifications of the Large Ribosome Subunit Assembly Intermediates inBiochemistry · 2025Article
- Arginine methylation of caspase-8 controls life/death decisions in extrinsic apoptotic networks.Oncogene · 2024Article
- 16S rRNA Methyltransferases as Novel Drug Targets Against Tuberculosis.The protein journal · 2022Review
- Nonessential tRNA and rRNA modifications impact the bacterial response to sub-MIC antibiotic stress.microLife · 2022Article
- Isolation of novel cold-tolerance genes from rhizosphere microorganisms of Antarctic plants by functional metagenomics.Frontiers in microbiology · 2022Article
- AtRsmD Is Required for Chloroplast Development and Chloroplast Function inFrontiers in plant science · 2022Article
- Fine-Mapping and Functional Analyses of a Candidate Gene Controlling Isoflavone Content in Soybeans Seed.Frontiers in plant science · 2022Article
- RNA methylation in chloroplasts or mitochondria in plants.RNA biology · 2021Review
- Review
- Complete genome sequence of high-yield strainSynthetic and systems biotechnology · 2020Article
- Grad-seq in a Gram-positive bacterium reveals exonucleolytic sRNA activation in competence control.The EMBO journal · 2020Article
- The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development.Nucleic acids research · 2020Article
- Comprehensive Functional Analysis ofFrontiers in genetics · 2020Article
- Structural and evolutionary insights into ribosomal RNA methylation.Nature chemical biology · 2018Review
- TRMT1-Catalyzed tRNA Modifications Are Required for Redox Homeostasis To Ensure Proper Cellular Proliferation and Oxidative Stress Survival.Molecular and cellular biology · 2017Article
- Identification of the methyltransferase targeting C2499 in Deinococcus radiodurans 23S ribosomal RNA.Extremophiles : life under extreme conditions · 2016Article
- Evolutionary and sequence-based relationships in bacterial AdoMet-dependent non-coding RNA methyltransferases.BMC research notes · 2014Article
- Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis.Nucleic acids research · 2014Article
- The nucleoid as a site of rRNA processing and ribosome assembly.Frontiers in plant science · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
N(2)-Methylguanine 966 is located in the loop of Escherichia coli 16 S rRNA helix 31, forming a part of the P-site tRNA-binding pocket. We found yhhF to be a gene encoding for m(2)G966 specific 16 S rRNA methyltransferase. Disruption of the yhhF gene by kanamycin resistance marker leads to a loss of modification at G966. The modification could be rescued by expression of recombinant protein from the plasmid carrying the yhhF gene. Moreover, purified m(2)G966 methyltransferase, in the presence of S-adenosylomethionine (AdoMet), is able to methylate 30 S ribosomal subunits that were purified from yhhF knock-out strain in vitro. The methylation is specific for G966 base of the 16 S rRNA. The m(2)G966 methyltransferase was crystallized, and its structure has been determined and refined to 2.05A(.) The structure closely resembles RsmC rRNA methyltransferase, specific for m(2)G1207 of the 16 S rRNA. Structural comparisons and analysis of the enzyme active site suggest modes for binding AdoMet and rRNA to m(2)G966 methyltransferase. Based on the experimental data and current nomenclature the protein expressed from the yhhF gene was renamed to RsmD. A model for interaction of RsmD with ribosome has been proposed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.