ArticleAnalytical biochemistry2007
Transchip: single-molecule detection of transcriptional elongation complexes.
Article in Analytical biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- DNA combing on low-pressure oxygen plasma modified polysilsesquioxane substrates for single-molecule studies.Biomicrofluidics · 2014Article
- Toward single-molecule optical mapping of the epigenome.ACS nano · 2014Article
- Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.Chemical reviews · 2013Review
- Single-molecule sequence detection via microfluidic planar extensional flow at a stagnation point.Lab on a chip · 2010Article
- Fluorescent marker for direct detection of specific dsDNA sequences.Analytical chemistry · 2009Article
- Imaging and analysis of transcription on large, surface-mounted single template DNA molecules.Analytical biochemistry · 2008Article
- Single-molecule studies of RNA polymerase: motoring along.Annual review of biochemistry · 2008Review
- Combining atomic force and fluorescence microscopy for analysis of quantum-dot labeled protein-DNA complexes.Journal of molecular recognition : JMRArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
A new single-molecule system, Transchip, was developed for analysis of transcription products at their genomic origins. The bacteriophage T7 RNA polymerase and its promoters were used in a model system, and resultant RNAs were imaged and detected at their positions along single template DNA molecules. The Transchip system has drawn from critical aspects of Optical Mapping, a single-molecule system that enables the construction of high-resolution ordered restriction maps of whole genomes from single DNA molecules. Through statistical analysis of hundreds of single-molecule template/transcript complexes, Transchip enables analysis of the locations and strength of promoters, the direction and processivity of transcription reactions, and the termination of transcription. These novel results suggest that the new system may serve as a high-throughput platform to investigate transcriptional events on a large genome-wide scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.