ArticleBMC microbiology2006
Identification of potential CepR regulated genes using a cep box motif-based search of the Burkholderia cenocepacia genome.
Article in BMC microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Regulation of type VI secretion systems at the transcriptional, posttranscriptional and posttranslational level.Microbiology (Reading, England) · 2023Review
- Recipient Cell Factors Influence Interbacterial Competition Mediated by Two Distinct Burkholderia dolosa Contact-Dependent Growth Inhibition Systems.Journal of bacteriology · 2022Article
- A LysR Family Transcriptional Regulator Modulates Burkholderia cenocepacia Biofilm Formation and Protease Production.Applied and environmental microbiology · 2021Article
- ScmR, a Global Regulator of Gene Expression, Quorum Sensing, pH Homeostasis, and Virulence inJournal of bacteriology · 2020Article
- Loss ofmSphere · 2019Article
- Article
- Immune Recognition of the Epidemic Cystic Fibrosis Pathogen Burkholderia dolosa.Infection and immunity · 2017Article
- VarR controls colonization and virulence in the marine macroalgal pathogen Nautella italica R11.Frontiers in microbiology · 2015Article
- Bioinformatic prediction of gene functions regulated by quorum sensing in the bioleaching bacterium Acidithiobacillus ferrooxidans.International journal of molecular sciences · 2013Article
- The unexpected discovery of a novel low-oxygen-activated locus for the anoxic persistence of Burkholderia cenocepacia.The ISME journal · 2013Article
- Identification of quorum sensing-controlled genes in Burkholderia ambifaria.MicrobiologyOpen · 2013Article
- Identification of functions linking quorum sensing with biofilm formation in Burkholderia cenocepacia H111.MicrobiologyOpen · 2012Article
- A unique regulator contributes to quorum sensing and virulence in Burkholderia cenocepacia.PloS one · 2012Article
- Saturation mutagenesis of a CepR binding site as a means to identify new quorum-regulated promoters in Burkholderia cenocepacia.Molecular microbiology · 2011Article
- The Burkholderia cenocepacia LysR-type transcriptional regulator ShvR influences expression of quorum-sensing, protease, type II secretion, and afc genes.Journal of bacteriology · 2011Article
- Nooks and crannies in type VI secretion regulation.Journal of bacteriology · 2010Review
- Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia.BMC genomics · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Burkholderia cenocepacia CepIR quorum sensing system has been shown to positively and negatively regulate genes involved in siderophore production, protease expression, motility, biofilm formation and virulence. In this study, two approaches were used to identify genes regulated by the CepIR quorum sensing system. Transposon mutagenesis was used to create lacZ promoter fusions in a cepI mutant that were screened for differential expression in the presence of N-acylhomoserine lactones. A bioinformatics approach was used to screen the B. cenocepacia J2315 genome for CepR binding site motifs.
resultsFour positively regulated and two negatively regulated genes were identified by transposon mutagenesis including genes potentially involved in iron transport and virulence. The promoter regions of selected CepR regulated genes and site directed mutagenesis of the cepI promoter were used to predict a consensus cep box sequence for CepR binding. The first-generation consensus sequence for the cep box was used to identify putative cep boxes in the genome sequence. Eight potential CepR regulated genes were chosen and the expression of their promoters analyzed. Six of the eight were shown to be regulated by CepR. A second generation motif was created from the promoters of these six genes in combination with the promoters of cepI, zmpA, and two of the CepR regulated genes identified by transposon mutagenesis. A search of the B. cenocepacia J2315 genome with the new motif identified 55 cep boxes in 65 promoter regions that may be regulated by CepR.
conclusionUsing transposon mutagenesis and bioinformatics expression of twelve new genes have been determined to be regulated by the CepIR quorum sensing system. A cep box consensus sequence has been developed based on the predicted cep boxes of ten CepR regulated genes. This consensus cep box has led to the identification of over 50 new genes potentially regulated by the CepIR quorum sensing system.
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