ArticleJournal of structural and functional genomics2006
Economical parallel protein expression screening and scale-up in Escherichia coli.
Article in Journal of structural and functional genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Implementation of Perforated Concentric Ring Walls Considerably Improves Gas-Liquid Mass Transfer of Shaken Bioreactors.Frontiers in bioengineering and biotechnology · 2022Article
- CK2 activates kinesin via induction of a conformational change.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer.Microbial cell factories · 2011Article
- Preparation of protein samples for NMR structure, function, and small-molecule screening studies.Methods in enzymology · 2011Article
- High-throughput expression and purification of membrane proteins.Journal of structural biology · 2010Article
- Structure of HIV-1 reverse transcriptase with the inhibitor beta-Thujaplicinol bound at the RNase H active site.Structure (London, England : 1993) · 2009Article
- Crystal structure of a novel dimeric form of NS5A domain I protein from hepatitis C virus.Journal of virology · 2009Article
- Protein production and purification.Nature methods · 2008Review
- Small-scale, semi-automated purification of eukaryotic proteins for structure determination.Journal of structural and functional genomics · 2007Article
- Production of selenomethionyl-derivatized proteins in baculovirus-infected insect cells.Protein science : a publication of the Protein Society · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel microfermentation and scale-up platform for parallel protein production in Escherichia coli is described. The vertical shaker device Vertiga, which generates low-volume high density (A(600) approximately 20) Escherichia coli cultures in 96-position deep-well plates without auxiliary oxygen supplementation, has been coupled to a new disposable shake flask design, the Ultra Yield flask, that allows for equally high cell culture densities to be obtained. The Ultra Yield flask, which accommodates up to 1 l in culture volume, has a baffled base and a more vertical wall construction compared to traditional shake flask designs. Experimental data is presented demonstrating that the Ultra Yield flask generates, on average, an equivalent amount of recombinant protein per unit cell culture density as do traditional shake flask designs but at a substantially greater amount per unit volume. The combination of Vertiga and the Ultra Yield flask provides a convenient and scalable low-cost solution to parallel protein production in Escherichia coli.
Indexed as
Identifiers
17187226What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.