ArticleBMC genomics2006
Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Cell Type-Specific Promoters ofGenes · 2023Article
- Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation.Scientific reports · 2021Article
- Different regulations of cell-type transcription by UV-B in multicellular green algaPlant signaling & behavior · 2019Article
- Cell-Type Transcriptomes of the Multicellular Green AlgaG3 (Bethesda, Md.) · 2018Article
- Whole transcriptome RNA-Seq analysis reveals extensive cell type-specific compartmentalization in Volvox carteri.BMC biology · 2017Article
- Volvox: A simple algal model for embryogenesis, morphogenesis and cellular differentiation.Developmental biology · 2016Review
- Comparative genomic analysis of retrogene repertoire in two green algae Volvox carteri and Chlamydomonas reinhardtii.Biology direct · 2016Article
- Characterization of Hydrogen Metabolism in the Multicellular Green Alga Volvox carteri.PloS one · 2015Article
- Cell-type specific photoreceptors and light signaling pathways in the multicellular green alga Volvox carteri and their potential role in cellular differentiation.Plant signaling & behavior · 2015Article
- Genome-wide analysis of alternative splicing in Volvox carteri.BMC genomics · 2014Article
- Cell-type specific light-mediated transcript regulation in the multicellular alga Volvox carteri.BMC genomics · 2014Article
- Review
- Cloning and Expression Profiling of the Polycomb Gene, Retinoblastoma-related Protein from Tomato Solanum lycopersicum L.Evolutionary bioinformatics online · 2014Article
- Validation of reference genes for quantitative gene expression studies in Volvox carteri using real-time RT-PCR.Molecular biology reports · 2013Article
- Factors controlling induction of reproduction in algae--review: the text.Folia microbiologica · 2012Review
- Building blocks of the nexin-dynein regulatory complex in Chlamydomonas flagella.The Journal of biological chemistry · 2011Article
- Channelrhodopsins of Volvox carteri are photochromic proteins that are specifically expressed in somatic cells under control of light, temperature, and the sex inducer.Plant physiology · 2009Article
- Cysteine modification of a specific repressor protein controls the translational status of nucleus-encoded LHCII mRNAs in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A gender-specific retinoblastoma-related protein in Volvox carteri implies a role for the retinoblastoma protein family in sexual development.The Plant cell · 2008Article
- Potential impact of gene regulatory mechanisms on the evolution of multicellularity in the volvocine algae.Communicative & integrative biologyArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe multicellular alga Volvox carteri possesses only two cell types: mortal, motile somatic cells and potentially immortal, immotile reproductive cells. It is therefore an attractive model system for studying how cell-autonomous cytodifferentiation is programmed within a genome. Moreover, there are ongoing genome projects both in Volvox carteri and in the closely related unicellular alga Chlamydomonas reinhardtii. However, gene sequencing is only the beginning. To identify cell-type specific expression and to determine relative expression rates, we evaluate the potential of real-time RT-PCR for quantifying gene transcript levels.
resultsHere we analyze a diversified pool of 39 target genes by real-time RT-PCR for each cell type. This gene pool contains previously known genes with unknown localization of cellular expression, 28 novel genes which are described in this study for the first time, and a few known, cell-type specific genes as a control. The respective gene products are, for instance, part of photosynthesis, cellular regulation, stress response, or transport processes. We provide expression data for all these genes.
conclusionThe results show that quantitative real-time RT-PCR is a favorable approach to analyze cell-type specific gene expression in Volvox, which can be extended to a much larger number of genes or to developmental or metabolic mutants. Our expression data also provide a basis for a detailed analysis of individual, previously unknown, cell-type specifically expressed genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.