ArticleThe EMBO journal2007
Insulin delays the progression of Drosophila cells through G2/M by activating the dTOR/dRaptor complex.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Rapamycin supplementation ofRoyal Society open science · 2023Article
- Systemic orchestration of cell size throughout the body: influence of sex and rapamycin exposure inBiology letters · 2023Article
- Trans-omics analysis of insulin action reveals a cell growth subnetwork which co-regulates anabolic processes.iScience · 2022Article
- Thermal and Oxygen Flight Sensitivity in AgeingBiology · 2021Article
- mTOR Signaling at the Crossroad between Metazoan Regeneration and Human Diseases.International journal of molecular sciences · 2020Review
- Nutrient restriction causes reversible G2 arrest inDevelopment (Cambridge, England) · 2019Article
- Modulation of the Proteostasis Machinery to Overcome Stress Caused by Diminished Levels of t6A-Modified tRNAs in Drosophila.Biomolecules · 2017Article
- Article
- Cell cycle progression is an essential regulatory component of phospholipid metabolism and membrane homeostasis.Open biology · 2015Article
- Mitochondrial Fragmentation Due to Inhibition of Fusion Increases Cyclin B through Mitochondrial Superoxide Radicals.PloS one · 2015Article
- RAD001 (everolimus) induces dose-dependent changes to cell cycle regulation and modifies the cell cycle response to vincristine.Oncogene · 2013Article
- Hepatocytes polyploidization and cell cycle control in liver physiopathology.International journal of hepatology · 2012Article
- Role of TOR signaling in aging and related biological processes in Drosophila melanogaster.Experimental gerontology · 2011Review
- Arabidopsis S6 kinase mutants display chromosome instability and altered RBR1-E2F pathway activity.The EMBO journal · 2010Article
- Mitotic raptor promotes mTORC1 activity, G(2)/M cell cycle progression, and internal ribosome entry site-mediated mRNA translation.Molecular and cellular biology · 2010Article
- A role for p38 stress-activated protein kinase in regulation of cell growth via TORC1.Molecular and cellular biology · 2010Article
- The insulin/Akt pathway controls a specific cell division program that leads to generation of binucleated tetraploid liver cells in rodents.The Journal of clinical investigation · 2009Article
- PDGF/VEGF signaling controls cell size in Drosophila.Genome biology · 2009Article
- Diet controls normal and tumorous germline stem cells via insulin-dependent and -independent mechanisms in Drosophila.Developmental biology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Drosophila and mammals, insulin signalling can increase growth, progression through G1/S, cell size and tissue size. Here, we analyse the way insulin affects cell size and cell-cycle progression in two haemocyte-derived Drosophila cell lines. Surprisingly, we find that although insulin increases cell size, it slows the rate at which these cells increase in number. By using BrdU pulse-chase to label S-phase cells and follow their progression through the cell cycle, we show that insulin delays progression through G2/M, thereby slowing cell division. The ability of insulin to slow progression through G2/M is independent of its ability to stimulate progression through G1/S, so is not a consequence of feedback by the cell-cycle machinery to maintain cell-cycle length. Insulin's effects on progression through G2/M are mediated by dTOR/dRaptor signalling. Partially inhibiting dTOR/dRaptor signalling by dsRNAi or mild rapamycin treatment can increase cell number in cultured haemocytes and the Drosophila wing, respectively. Thus, insulin signalling can influence cell number depending on a balance between its ability to accelerate progression through G1/S and delay progression through G2/M.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.