ArticleMolecular biology of the cell2007
GCP6 binds to intermediate filaments: a novel function of keratins in the organization of microtubules in epithelial cells.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- Human testis-specific TTRP regulates circadian clock as an evolutionarily young natural antisense transcript of PER2.Nature communications · 2026Article
- Searching for protein partners of short-chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD) reveals keratin 8 as a novel candidate for interaction in pancreatic β-cells.BMC molecular and cell biology · 2025Article
- Article
- Dual-color live imaging unveils stepwise organization of multiple basal body arrays by cytoskeletons.EMBO reports · 2024Article
- A germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans.Journal of cell science · 2023Article
- γ-Tubulin in microtubule nucleation and beyond.Frontiers in cell and developmental biology · 2022Review
- Molecular insight into how γ-TuRC makes microtubules.Journal of cell science · 2021Review
- Review
- Microtubule nucleation by γ-tubulin complexes and beyond.Essays in biochemistry · 2018Review
- PLK4 is a microtubule-associated protein that self-assembles promotingJournal of cell science · 2018Article
- Assembly and regulation of γ-tubulin complexes.Open biology · 2018Review
- Microtubule organization, dynamics and functions in differentiated cells.Development (Cambridge, England) · 2017Review
- Regulation of microtubule nucleation mediated by γ-tubulin complexes.Protoplasma · 2017Review
- Functional Analysis of γ-Tubulin Complex Proteins Indicates Specific Lateral Association via Their N-terminal Domains.The Journal of biological chemistry · 2016Article
- Review
- Review
- Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat-Shock Protein Chaperoning and Kinase Rescue.Methods in enzymology · 2016Article
- Microtubule-organizing center-mediated nuclear polarity in various normal and neoplastic human tissues.Virchows Archiv : an international journal of pathology · 2015Article
- Organization and execution of the epithelial polarity programme.Nature reviews. Molecular cell biology · 2014Review
- Keratins and protein synthesis: the plot thickens.The Journal of cell biology · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In simple epithelial cells, attachment of microtubule-organizing centers (MTOCs) to intermediate filaments (IFs) enables their localization to the apical domain. It is released by cyclin-dependent kinase (Cdk)1 phosphorylation. Here, we identified a component of the gamma-tubulin ring complex, gamma-tubulin complex protein (GCP)6, as a keratin partner in yeast two-hybrid assays. This was validated by binding in vitro of both purified full-length HIS-tagged GCP6 and a GCP6(1397-1819) fragment to keratins, and pull-down with native IFs. Keratin binding was blocked by Cdk1-mediated phosphorylation of GCP6. GCP6 was apical in normal enterocytes but diffuse in K8-null cells. GCP6 knockdown with short hairpin RNAs (shRNAs) in CACO-2 cells resulted in gamma-tubulin signal scattered throughout the cytoplasm, microtubules (MTs) in the perinuclear and basal regions, and microtubule-nucleating activity localized deep in the cytoplasm. Expression of a small fragment GCP6(1397-1513) that competes binding to keratins in vitro displaced gamma-tubulin from the cytoskeleton and resulted in depolarization of gamma-tubulin and changes in the distribution of microtubules and microtubule nucleation sites. Expression of a full-length S1397D mutant in the Cdk1 phosphorylation site delocalized centrosomes. We conclude that GCP6 participates in the attachment of MTOCs to IFs in epithelial cells and is among the factors that determine the peculiar architecture of microtubules in polarized epithelia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.